血浆miR-193a-5p在急性髓系白血病诊疗评估中的价值  

Value of plasma miR-193a-5p level on diagnosis and treatment evaluation in acute myeloid leukemia

作　　者：张娜[1] 徐智芳 任方刚 赵俊霞[3] 许晶[3] 陈秀花 覃艳红 常建梅 薛凤[3] 高峰[3] 潘杰[3] 尹彬[3] 王宏伟 

机构地区：[1]山西医科大学第二医院检验科,太原030001 [2]血液病研究所血液病分子诊疗山西省重点实验室 [3]山西医科大学研究生学院,太原030001

出　　处：《白血病．淋巴瘤》2016年第6期349-353,共5页Journal of Leukemia & Lymphoma

基　　金：国家自然科学基金(81241014)；山西省回国留学人员重点科研资助项目(2015-重点5)；山西医科大学科技创新基金(01201413)Fund program:National Natural Science Foundation of China，Scientific Research Foundation for the Returned Overseas Chinese Scholars of Shanxi Province，Science and Technology Innovation Fund of Shanxi Medical University

摘　　要：目的探讨血浆miR-193a-5p在急性髓系白血病（AML）诊断和病程监测中的价值。方法选取2015年7月至11月山西医科大学第二医院血液科住院的AML患者，其中初发患者30例，完全缓解（CR）患者9例，复发患者6例，按简单随机抽样方法随机选取15名健康人作为健康对照。收集患者和健康人EDTA抗凝的外周血标本，分离血浆，提取RNA，应用实时荧光定量聚合酶链反应（qRT．PCR）检测血浆miR-193a-5p的表达水平。结果AML患者组血浆miR-193a-5p相对表达水平[0．4656（0．1031～5．0002）]低于健康对照组[0．7661（0．0521—3．1344）]（U=122，P=0．0187）。AML初发组和复发组患者血浆miR-193a-5p表达水平比CR组和健康对照组低（均P〈0．05），CR组和健康对照组之间表达水平差异无统计学意义（U=56，P=0．5119）。AML患者血浆miR-193a-5p表达水平与患者年龄、性别、骨髓原始细胞比例、外周血白细胞计数、血小板计数、CD34＋细胞比例等均无相关性（均P〉0．05）。结论血浆miR-193a-5p在AML患者中表达水平降低，可作为AML诊断及疗效评估的新型无创指标。Objective To explore the value of the plasma miR-193a-5p level on diagnosis and monitoring the response to treatment in acute myeloid leukemia （AML）. Methods Peripheral blood samples were obtained from AML patients enrolled in hematology department of the Second Hospital of Shanxi Medical University from July 2015 to December 2015, including 30 de novo AML patients, 9 patients in complete remission （CR） and 6 patients in relapse. Peripheral blood samples from 15 healthy people were randomly ehoosed as the health control group. Plasma miR-193a-5p expression levels were detected by using quantitative real-time reverse transcription polymerase chain reaction （qRT-PCR）. Results The plasma miR-193a-5p relative expression level of AML patients group [0.465 6 （0.103 1-5.000 2）] was obviously lower than that of health control group [0.766 1 （0.052 1-3.134 4）] （U = 122, P = 0.018 7）. The plasma miR-193a-5p relative expression levels of de novo group and relapse AML group were significantly lower than those of CR group and health control group （P 〈 0.05）, and there was no significant difference between the CR group and health control group （U = 56, P = 0.511 9）. No significant correlation was found between the plasma miR-193a-5p level and age, gender, blast percentage of the bone marrow, peripheral blood leukocyte count, platelet count, CD34＋ cells＂ percentage and so on. Conclusion The decreased plasma miR-193a-Sp expression level can be served as a new and noninvasive biomarker for the evaluation of diagnosis and treatment in AML.

关 键 词：白血病 髓样 急性 miR-193a-5p 聚合酶链反应 

分 类 号：R733.71[医药卫生—肿瘤]