软肝冲剂对四氯化碳致小鼠肝损伤的保护作用  

Protection effect of Ruangan Granule on carbon tetrachloride-induced liver damage in mice

作　　者：王良君[1] 杨亮[1] 

机构地区：[1]辽宁医学院卫生学教研室,辽宁锦州121001

出　　处：《中国工业医学杂志》2016年第3期177-181,F0003,共6页Chinese Journal of Industrial Medicine

摘　　要：目的观察软肝冲剂对四氯化碳(CCl4)诱导的小鼠肝纤维化模型的保护作用。方法将60只C57B/L小鼠随机分为空白对照组、模型组、秋水仙碱对照组、软肝冲剂低剂量组和软肝冲剂高剂量组,每组12只。空白对照组腹腔注射100%花生油1 ml/kg,每周2次,共10周;其余各组均给予腹腔注射含20%CCl4的花生油1 ml/kg,每周2次,共10周造模;第3周起秋水仙碱对照组予秋水仙碱0.1 mg/(kg·d)灌胃,软肝冲剂低、高剂量组分别予软肝冲剂1 g/(kg·d)、5 g/(kg·d)灌胃。在第10周通过摘除眼球取血,检测小鼠血清中透明质酸(HA)、层粘连蛋白(LN)、三型前胶原(PCⅢ)、四型胶原(Ⅳ-C)、丙氨酸转氨酶(ALT)、天门冬氨酸转氨酶(AST)含量,检测肝脏组织中超氧化物歧化酶(SOD)和丙二醛(MDA)的含量,计算肝脏指数,观察小鼠肝脏的HE染色病理组织学改变。结果模型组小鼠血清中HA、LN、PCⅢ、Ⅳ-C、ALT和AST含量分别为(480.11±62.12)μg/L、(411.65±30.63)μg/L、(454.12±41.81)μg/L、(311.15±31.61)μg/L、(140.4±9.8)U/L和(198.2±9.7)U/L,均明显高于其它各组(P<0.05);肝脏组织中SOD[(122.53±7.68)U/mg]低于空白对照组(P<0.05);MDA[(5.59±0.33)nmol/mg]高于空白对照组(P<0.05);肝脏指数[(4.7±1.5)%]高于空白对照组(P<0.05)。病理切片显示肝细胞坏死并出现脂肪空泡、纤维组织增生和炎细胞浸润。软肝冲剂低剂量组与秋水仙碱组治疗效果相似。软肝冲剂高剂量组效果较好,小鼠血清中HA、LN、PCⅢ、Ⅳ-C、ALT和AST含量分别为(143.21±21.53)μg/L、(139.11±35.23)μg/L、(134.45±24.77)μg/L、(119.32±29.34)μg/L、(45.4±6.3)U/L和(53.6±7.1)U/L,均明显低于模型组(P<0.05);肝脏组织中SOD[(189.5±7.5)U/mg]高于模型组(P<0.05),MDA[(2.45±0.33)nmol/mg]低于模型组(P<0.05);肝脏指数[(3.4±0.4)%]低于模型组(P<0.05);病理检查显示肝脏组织中无炎性浸润、脂肪空泡和无纤维组织沉积。结论软肝冲剂可以改善肝脏功能,保Objective To observe the protection effect or Ruangan Granule （RGG） on carbon tetrachloride induced-liver fibrosis in mice. Methods 60 C57B/L mice were randomly divided into five groups including control group, model group, eolehicine group, RGG low dose group and RGG high dose group, with 12 mice in each group. Mice in control group were intraperitoueally injected with 100% peanut oil 1 ml/kg twice a week for 10 weeks; mice in rest groups were all intraperitoneally injected with peanut oil containing 20% carbon tetrachloride 1 ml/kg, twice a week, then colchicines [ 0. 1 mg/（ kg·d） ], RGG [ 1 g/（ kg·d）] and RGG[ 5 g/（ kg·d）] were intragastrically administered for mice in colchicine group, low dose RGG group and high dose RGG group, respectively from third week. At the tenth week, the administrations were ended and the blood samples were collected by removing eyes method, and the contents of hyaluronie acid （HA） , laminin （LN） , three former Ⅲ （ PC Ⅲ ） , collagen-Ⅳ （Ⅳ-C） , alanine aminotransferase （ALT） and aspartate aminotransferase （AST） in serum were measured, meanwhile, the contents of super oxide dismutase （SOD） and malondialdehyde （MDA） in liver tissue were also tested, the liver index was calculated and pathological changes of liver were observed by HE staining as well. Results The results showed that in model group, the values of HA, LN, PC Ⅲ, Ⅳ-C, ALT and AST in serum were（480. 11±62. 12） μg/L, （411.65±30. 63）μg/L, （454. 12±41.81 ） μg/L, （311. 15±31.61 ） μg/L, （ 140. 4±9. 8） U/L and（ 198.2±9. 7） U/L, respectively, signifieantly higher than those in other groups （P〈0, 05）, the SOD level in liver tissue （122, 53±7.68） U/rag, was significantly lower than those in other groups （P〈0. 05）, the MDA level in liver tissue （5.59±0. 33） nmol/mg, was signifieantly higher than that in control group （P〈0.05）, the liver index （4. 7±1.5） %, was significantly higher than that in controis group

关 键 词：软肝冲剂 肝纤维化 超氧化物歧化酶(SOD) 丙二醛(MDA) 

分 类 号：R99[医药卫生—毒理学] O622.2[医药卫生—药学]