果蔬产品中甲型肝炎病毒风险评估和基因分型研究  

Risk Assessment and Genotyping of Hepatitis A Virus in Fruit and Vegetable Products

作　　者：房保海[1] 岳志芹[1] 孙涛[1] 梁成珠[1] 赵玉然[1] 林超[1] 郑小龙[1] 王群[1] 孙明君[1] 

机构地区：[1]山东出入境检验检疫局检验检疫技术中心,山东青岛266002

出　　处：《病毒学报》2016年第4期484-489,共6页Chinese Journal of Virology

基　　金：国家质检公益项目(项目号:201410080);题目:进出口果蔬检验检疫关键技术标准及风险分析研究;国家质检总局项目(项目号:2014IK024);题目:冷冻草莓诺如病毒和甲肝病毒风险评估

摘　　要：本研究探讨了果蔬产品中甲肝病毒风险评估和基因分型;采用实时荧光RT-PCR对216个果蔬样品进行了检测,检测出6个阳性样品,分别为冷冻草莓、冷冻蓝莓、冷冻土豆丁、冷冻苹果丁和冷冻树莓样品,占总样品数的2.8%;采用巢式RT-PCR对6个阳性样品进行基因扩增,样品冷冻苹果丁(210-1999)和冷冻蓝莓(210-2002)获得单一条带,经系统进化和同源性分析,二者皆属于甲肝病毒ⅠB亚型;本研究为果蔬产品企业和食品安全管理部门提供了此类产品甲肝病毒污染的风险信息,奠定病毒基因溯源基础,为企业从种植、收获、加工、包装等各关键控制点保证产品安全提供技术支撑,为流行病学诊断提供可靠数据。This study explored risk assessment and genotyping of hepatitis A virus（HAV）in fruit and vegetable products.Two hundred and sixteen samples of fruit and vegetable products were examined by realtime RT-PCR.Six samples tested positive for hepatitis A virus,including frozen strawberries,frozen blueberries,frozen diced potatoes,frozen diced apple and frozen raspberries,accounting for 2.8% of the total samples tested.These six HAV isolates were genotyped by nested RT-PCR amplification,and a single band was detected in isolates from frozen diced apple（210-1999）and frozen blueberries（210-2002）.These two isolates belong to the HAV IB subtype,based on analysis of evolution and homology.This study provides HAV risk information for fruit and vegetable enterprises and food safety management departments.Furthermore,it lays a foundation for HAV traceability,and provides technical support to ensure product safety for enterprises at critical control points including planting,harvest,processing and packaging.These results provide reliable data for epidemiological diagnosis.

关 键 词：甲型肝炎病毒(HAV) 果蔬产品 风险评估 基因分型 

分 类 号：R373.21[医药卫生—病原生物学]