I-TAC及其受体CXCR3在胃癌肝转移中的作用及临床意义  

Expression and clinical signiifcance of I-TAC and its receptor CXCR3 in gastric hepatic metastases

作　　者：董霞[1] 孙辉[1] 赵琨[1] 单新芳 李延海[2] 王杰书[1] 

机构地区：[1]山东省滨州医学院附属中心医院普外科,251700 [2]滨州医学院附属医院胃肠外科

出　　处：《中国临床实用医学》2016年第3期3-6,共4页China Clinical Practical Medicine

基　　金：山东省中医药科技发展计划（2015-498）

摘　　要：目的检测人干扰素诱导T细胞α亚族趋化因子（I-TAC）及CXC趋化因子受体3（CXCR3）在胃腺癌组织中的表达，并初步分析其在胃癌肝转移中的作用及意义。方法选取2013年1月至2016年1月滨州医学院附属中心医院手术切除的新鲜胃癌组织（伴肝转移）标本30例作为研究组，另择同期胃癌（不伴有肝转移）手术标本35例为对照组。采用逆转录聚合酶链反应技术、免疫组织化学技术检测两组标本中I-TAC及CXCR3的表达水平；并进一步分析上述两者的表达水平与常见临床病理参数的关系。结果研究组中I-TAC及CXCR3的mRNA和蛋白质表达水平均较对照组高。I-TAC在研究组中阳性表达率（93.33%）高于对照组中阳性表达率（31.43%），两组差异有统计学意义（P=0.007）。CXCR3在研究组中阳性表达率（83.33%）高于对照组中阳性表达率（45.71%），比较差异亦有统计学意义（P=0.015）。相关性分析表明，CXCR3的表达与胃癌组织分化程度、淋巴结转移等呈正相关。结论伴肝转移胃癌组织中存在I-TAC及其受体CXCR3的特异性激活，提示I-TAC/CXCR3信号传导通路在胃癌肝转移过程中发挥重要作用。Objective To investigate the expression of I-TAC、CXCR3 in gastric cancer, and to reveal the role of I-TAC/CXCR3 in the metastasis of gastric carcinoma. Methods Two groups were selected,the research group （30 gastric cancer tissues with hepatic metastasis）,and the control group （35 gastric cancer tissues without liver metastasis）. Expression of I-TAC、CXCR3 in surgical specimens were determined by reverse transcription-polymerase chain reaction and immunohistochemistry, respectively. The expression of I-TAC and CXCR3 was detected by immunohistochemical staining in surgical specimens of gastric carcinoma with and without hepatic metastasis. Associations between the above two indicators and clinicopathological characteristics were finally analyzed. Results Compared with those without liver metastasis of gastric cancer tissue,the gene expression of I-TAC and CXCR3 in the gastric carcinoma tissues with hepatic metastasis were significantly higher. Positive expression rate of I-TAC in the gastric tissues with hepatic metastasis were 93.33%%, while positive expression rate of I-TAC in the gastric tissues without hepatic metastasis were 31.43%%, which were significantly lower than that of the group with hepatic metastasis. Positive expression rate of CXCR3 in the research group were 83.33%, while the positive expression rate of CXCR3 in the control group were 45.71%, which were significantly lower than that of the group with hepatic metastasis. Expression of CXCR3 in the tissue of gastric carcinoma were positively related to tissue histologic differentiation, lymph node metastasis. Conclusion Up-regulated expression of I-TAC＆amp;nbsp;and CXCR3 was associated with gastric cancer metastasis, I-TAC-CXCR3 signaling pathway may play a role in the process of gastric hepatic metastases.

关 键 词：人干扰素诱导T细胞α亚族趋化因子(I-TAC) CXC趋化因子受体3(CXCR3) 趋化因子 胃癌肝转移 CXCR3 

分 类 号：R735[医药卫生—肿瘤]