胃癌不同证型与化疗敏感性研究  

Different Syndrome Types and Gastric Cancer Chemotherapy Sensitivity

作　　者：王冰[1] 杨宇 臧文巧[3] 王涛[1] 关徐涛[1,4] 孙士玲[5] 丁璐[5] 高萍[1] 

机构地区：[1]河南中医学院第一附属医院血液肿瘤科,河南郑州450000 [2]商丘市中医院肿瘤科,河南商丘450000 [3]郑州大学基础医学院,河南郑州450000 [4]山东中医药大学 [5]河南中医学院,河南郑州450000

出　　处：《中华中医药学刊》2016年第7期1565-1568,共4页Chinese Archives of Traditional Chinese Medicine

基　　金：国家自然科学基金项目(81301726)

摘　　要：目的:胃癌是我国常见的恶性肿瘤,死亡率高,胃癌的治疗仍以化疗为主,而长期化疗使得患者产生耐药,影响化疗效果。中西医结合治疗肿瘤受到关注,本研究将60例患者在术前进行中医辨证,用MTT法测定不同证型患者胃癌细胞体外药物的敏感性并探讨其相关性。方法:将60例原发性胃癌患者手术前进行辨证,术后将癌组织送至实验室提取出胃癌细胞,超低温保存,利用MTT法测定5种化疗药物针对不同中医证型胃癌患者的体外敏感性。并计算其抑制率。抑制率采用均数±标准差(±s)表示,组内数据的比较采用两因素的方差分析,组间两两比较采用重复测量方差分析F检验。当P<0.05具有统计学差异。以上数据均采用SPSS 19.0统计软件处理。结果:5种化疗药物对60例胃癌患者在细胞体外药物敏感性上均无显著性差异(P>0.05)。5种化疗药物在抑制率上气血亏虚组表现为不敏感甚至耐药,两两证型相比气血亏虚组与肝胃不和组以及痰食瘀阻组比较有显著性差异(P<0.05)结论:不同证型胃癌患者对5种化疗药物敏感性有相关性,肝胃不和最敏感,依次为脾胃虚寒、痰食瘀阻,气血亏虚最差。Objective： Gastric cancer is a kind of common malignant tumor in China. The mortality rate is high. The treatment is still chemotherapy,while patients with long- term chemotherapy can occasionally result in drug resistance,affecting the effect of chemotherapy. Combining traditional Chinese and western medicine treatment of the tumor,this study gave TCM syndrome differentiation of 60 patients before operation and by MTT method tested different syndrome types＇ drug sensitivity in vitro and its correlation. Method： A total of 60 patients with primary gastric cancer before surgery were made syndrome differentiation. After operation,we extracted the gastric cancer cells and measured the sensitivity in vitro of 5 kinds of chemotherapy drugs by the MTT method. And their inhibition rate was calculated. Two factors analysis of variance was used intra- group and between two groups F test. P〈0. 05 for the difference was statistically significant.SPSS 19. 0 was used. Results A total of 5 kinds of chemotherapy drugs sensitivity in 60 cases of gastric cancer cells in vitro had no significant difference（ P〉0. 05）. Inhibition rate of 5 types of chemotherapy drugs in the deficiency of qi and blood group was not sensitive and even resistance. Deficiency of qi and blood and liver- stomach disharmony group and phlegm and blood stasis resistance groups had significant difference（ P〈0. 05）. Conclusion Different syndrome types of gastric cancer patients has relevance to the five kinds of chemotherapeutic drugs sensitivity. Liver- stomach disharmony syndrome had the most sensitive,followed by spleen and stomach cold,phlegm and blood stasis and qi deficiency syndrome is the worst.

关 键 词：胃癌 辨证分型 化疗敏感性 

分 类 号：R735.2[医药卫生—肿瘤]