四妙勇安汤抑制血管内皮细胞p-选择素、NF-κB表达的实验研究  

Simiao Yong'an Decoction Inhibiting Expressions of p- selectin and NF-κB in Vascular Endothelial Cells

作　　者：李光辉[1] 张军平[2] 丁义[2] 曹阳[1] 董玮[2] 彭立[2] 

机构地区：[1]天津市武清区中医医院,天津301700 [2]天津中医药大学第一附属医院心血管内科,天津300193

出　　处：《中华中医药学刊》2016年第7期1613-1615,共3页Chinese Archives of Traditional Chinese Medicine

基　　金：国家自然科学基金项目(30901904);天津市自然科学基金重点项目(09JCZDJC20700)

摘　　要：目的:探讨四妙勇安汤及其单体绿原酸、阿魏酸对于凝血酶处理的内皮细胞P-选择素及NF-κb p65mRNA表达的影响。方法:MTT法检测绿原酸、阿魏酸、四妙勇安汤对于内皮细胞活力的影响;0.2 U/mL凝血酶处理内皮细胞15 min作为模型组,三种药物预处理内皮细胞24 h作为干预组,流式细胞术、Elisa检测内皮细胞P-选择素的表达,RT-PCR检测NF-κb p65mRNA的表达。结果:绿原酸102ng/m L、阿魏酸104ng/mL时OD值最大;模型组P-选择素及NF-κb p65mRNA的表达均高于空白组(P<0.05),各干预组P-选择素及NF-κb p65mRNA的表达均低于模型组,组间无差异,但四妙组数值低于其余两组。结论:四妙勇安汤及其单体能减少内皮细胞P-选择素的表达,这一作用可能是通过抑制NF-κb p65mRNA的表达来完成的。Objective： To investigate the influence of Simiao Yong＇an Decoction and its monomer green chlorogenic acid and ferulic acid on the expressions of endothelial cell P- selectin and NF- κb p65 m RNA. Methods： MTT assay was used to test the green chlorogenic acid,ferulic acid and Simiao Yong＇an Decoction＇s influence on endothelial cell viability. The endothelial cells treated with 0. 2 U / m L thrombin for 15 min were as the model group. The endothelial cells which were given three drugs pretreatment for 24 h were as intervention groups. Flow cytometry and ELISA were used to detect endothelial cell P- selectin expression and RT- PCR to detect the expression of NF- κb p65 m RNA. Results：When chlorogenic acid was 102 ng / m L and the ferulic acid 104 ng / m L,OD values were the maximum. The model group＇s P- selectin and NF- κb p65 m RNA expressions were higher than those of the blank group（ P〈0. 05）. Each intervention group＇s P- selectin and NF- κb p65 m RNA expressions were lower than the model group＇s. There were no differences among groups but the value was lower than the other two groups＇. Conclusion： Simiao Yong＇an Decoction and its monomer can reduce P- selectin expression in endothelial cells,which might be accomplished by inhibiting the expression of NF- κb p65 m RNA.

关 键 词：四妙勇安汤 绿原酸 阿魏酸 P-选择素 NF-ΚB p65mRNA 

分 类 号：R285.5[医药卫生—中药学]