DHP-半乳糖复合体制备多孔生物载体及其在人体肝细胞培养中的应用  

Preparation of Porous Biological Carrier with DHP-galactose Complex and Its Application in Culture of Human Hepatocytes

作　　者：吴晨曦[1] 谢益民[1,2] 叶哲孜 王鹏[1,2] 乐喜 

机构地区：[1]湖北工业大学制浆造纸工程学院,武汉430068 [2]湖北工业大学绿色轻工材料湖北省重点实验室,武汉430068

出　　处：《生物技术通报》2016年第6期250-257,共8页Biotechnology Bulletin

基　　金：国家自然科学基金项目(31370574;31300494);湖北省教育厅科学研究计划资助项目(Q20131402);湖北工业大学高层次人才基金资助(BSQD12037);绿色轻工材料湖北省重点实验室开放基金[省重点实验室科(2013)2号-面上]

摘　　要：为了使木素脱氢聚合物(DHP)与人体肝细胞之间有更好的生物相容性,使用DHP和D-半乳糖进行接枝共聚,通过水凝胶的方法制备医用多孔生物载体。利用红外光谱、核磁共振波谱、比表面积测定仪及扫描电子显微镜对多孔生物载体的主要成分含量、物理和化学结构以及表面形态进行分析,并对体外培养中的肝细胞形态与代谢活性进行检测。结果表明,以DHP与D-半乳糖的接枝共聚产物作为原料可以制备性能良好的多孔生物载体,比表面积为6.013 m2/g。使用此多孔生物载体对人体肝细胞进行培养,人体肝细胞能够健康地附着在DHP多孔生物载体上生长增殖。根据含半乳糖的生物载体白蛋白分泌和葡萄糖代谢结果,最大值分别为:9.85 g/(d·L)、16.134 mmol/(d·L),说明肝细胞在培养阶段中具有较高的代谢活性,DHP与D-半乳糖的接枝共聚产物制备的多孔生物载体具有良好的生物相容性。In order to enhance the biocompatibility of dehydrogenation polymer （ DHP ） with human hepatocytes, the DHP was copolymerized with D-galactose, by which a porous biological carrier for medical use was prepared using the method of hydrogel. Fourier transform infrared spectrometer （ FT-IR ）, nuclear magnetic resonance （ NMR ） spectroscopy, BET specific surface area determination, and scanning electron microscope （ SEM ） were applied to elucidate the main composition, chemical and physical structure, and morphology of the porous biological carrier. Inverted microscope and albumin and glucose kits were used to check the morphology and metabolic activity of cultured hepatocytes. The results indicated that the porous biological carrier with excellent performance was prepared with co-polymer of DHP and D-galaetose as raw material, and the specific surface area was 6.013 m2/g. The human hepatocytes healthily adhered on the porous biological carrier to grow and proliferate while the carriers were used to culture human hepatocytes. The maximum values of albumin secretion and glucose consumption using biological carrier containing galactose were 9.85 g/（ d · L ） and 16.134 mmol/（ d · L ）, respectively, demonstrating that the hepatocytes presented high metabolic activity during culturing, and the porous biological carrier prepared with DHP-galactose complex had a great biocompatibility.

关 键 词：DHP 生物载体 人体肝细胞 细胞培养 生物相容性 

分 类 号：R575[医药卫生—消化系统] R318.08[医药卫生—内科学]