抑郁症患者血浆microRNA-16与microRNA-195表达水平的研究  

Expression levels of plasma microRNA-16 and microRNA-195 in depression patients

作　　者：冯倩[1] 汤臻[2] 周华[1] 孙沁怡[1] 朱浩[1] 邱晨红[1] 周沁[1] 赵中[1] 

机构地区：[1]南京医科大学附属苏州医院神经内科,苏州215000 [2]苏州市广济医院临床心理科,苏州215001

出　　处：《中华行为医学与脑科学杂志》2016年第6期525-529,共5页Chinese Journal of Behavioral Medicine and Brain Science

基　　金：苏州市科技发展计划项目（SYS201426）

摘　　要：目的 了解血浆microRNA-16（miR-16）,microRNA-195（miR-195）在抑郁症及抗抑郁治疗中的表达情况,初步探讨miRNA-16、miRNA-195在抑郁症临床应用的可能性.方法 收集60例初诊抑郁症患者（研究组）和30例健康志愿者（对照组）,收集两组人口学资料,对研究组和对照组用汉密顿抑郁量表（HAMD）进行评分.将研究组随机分为两组,每组各30例,一组（A组）给予5-羟色胺再摄取抑制剂（氟西汀）抗抑郁治疗,另一组（B组）给予双通道抑制剂（文拉法辛）抗抑郁治疗,经过6周抗抑郁治疗后,再次采集患者外周血,采用实时荧光定量PCR方法检测上述血浆中miRNA-16,miRNA-195水平.分析两种miRNAs在不同组间的相对表达情况.结果 A、B两组治疗前血浆miRNA-16及miRNA-195水平差异无统计学意义（t=0.536,P＞0.05;t=0.212,P＞0.05）.A、B两组治疗前后血浆miRNA-16表达水平较对照组均降低（A组：t0=-6.609、t6=-4.755,P＜0.01;B组：t0=-7.171、t6-5.149,P＜0.01）;A、B两组治疗前后血浆miRNA-195表达水平较对照组均降低（A组：t0=-6.626、t6=-6.379,P＜0.01;B组：t0=-6.690、t6=-6.525,P＜0.01）.A、B两组抗抑郁治疗6周后HAMD值较治疗前明显降低（t=46.116、46.142,P＜0.01）.A、B两组经抗抑郁治疗6周后血浆miRNA-16和miRNA-195表达水平均较未治疗时升高（miRNA-16：tA=-3.366、tB=-3.299,P＜0.05;miRNA-195：tA=-5.646、tB=-4.081,P＜0.05）.结论 血浆miRNA-16、miRNA-195的表达水平降低与抑郁症发生相关,经抗抑郁治疗,miRNA-16与miRNA-195水平较前有显著提高,miRNA-16与miRNA-195有可能成为抑郁症的筛查及抗抑郁疗效的评估指标.Objective To investigate expression levels of plasma miRNA-16 and miRNA-195 iu pre-and post-treatment depression patients,and to explore the clinical application possibility of miRNA-16 and miRNA-195 in the premliminary.Methods 60 cases of depression patients （research group） and 30 cases of healthy volunteers （control group） were collected,then the information and HAMD scores of research research group and control group were detected.The research group were randomly divided into two groups,each group 30 cases,one group （group A） were given serotonin reuptake inhibitors （fluoxetine） as antidepressant treatment,another group （group B） were given the dual channel inhibitors （venlafaxine） as antidepressant treatment.After 6 weeks＇ treatment,patients＇ peripheral blood were collected again.The levels of miRNA-16 and miRNA-195 were detected in plasma using real-time fluorescent quantitative PCR method.Then the levels of the two miRNAs in different groups were analyzed.Results There was no difference between group A and group B of plasma miRNA-16 level without treatment,also of plasma miRNA-195 （t=0.536,0.212,P〉0.05）.Before and after treatment,plasma miRNA-16 levels of group A and group B were significantly lower than that of control group（group A t0=-6.609,t6=-4.755,P〈0.01 ;group B t0=-7.171,t6=-5.149,P〈0.01）.Before and after treatment,plasma miRNA-195 levels of group A and group B were significantly lower than that of control group（group A t0=-6.626,t6=-6.379,P〈0.01 ;group B t0=-6.690,t6=-6.525,P〈0.01）.After 6 weeks＇ antidepressant treatment,the scores of HAMD were significantly lower than pre-treatment in both group A and group B,and the difference was statistically significant （t=46.116,46.142,P〈0.01）.After 6 weeks＇ antidepressant treatment,the levels of plasma miRNA-16 and miRNA-195 elevated compared with pre-treatment in group A and group B（miRNA-16 tA=-3.366,tB=-3.299,P〈0.05 ;miRNA-195 tA=-5.646,tB=-4.081,P〈0.05）.The scores of HAMD were not corre

关 键 词：MicroRNA-16 MicroRNA-195 抑郁症 

分 类 号：R749.4[医药卫生—神经病学与精神病学]