Label-free quantification of peptides in solution by disposable patterned hydrophilic chip based MALDI imaging  

作　　者：Ting Wu Xiao-Hui Yang Chuan-Jing Zhang Zhen-Ping Wang Yi-Ping Du 

机构地区：[1]Shanghai Key Laboratory of Functional Materials Chemistry, Research Centre of Analysis and Test, East China University of Science and Technology

出　　处：《Chinese Chemical Letters》2016年第6期901-904,共4页中国化学快报（英文版）

基　　金：supported by the National Natural Science Foundation of China (No. 21205041);the Fundamental Research Funds for the Central Universities (No. 222201314039);a grant from Shanghai Municipal Education Committee (No. YJ0114209)

摘　　要：Quantification of a mixture of peptides in solution was achieved by disposable patterned hydrophilic chip based matrix-assisted laser desorption/ionization mass spectrometric imaging（MALDI MSI）.Compared with other quantitative methods for peptides in solution, this method is label-free and does not require separation of the multiple components of the solution before analysis. Uniform hydrophilic spots and high mass accuracy measurements provided confident identification and quantitative analysis of imaged compounds. The linear correlation between concentration and grayscale of image in the range of 5 fmol/μ L to 1 pmol/μ L was obtained for all four peptides. Good sensitivity and excellent reproducibility were also achieved. The method expands the application of MALDI MSI from tissues to solutions.Quantification of a mixture of peptides in solution was achieved by disposable patterned hydrophilic chip based matrix-assisted laser desorption/ionization mass spectrometric imaging（MALDI MSI）.Compared with other quantitative methods for peptides in solution, this method is label-free and does not require separation of the multiple components of the solution before analysis. Uniform hydrophilic spots and high mass accuracy measurements provided confident identification and quantitative analysis of imaged compounds. The linear correlation between concentration and grayscale of image in the range of 5 fmol/μ L to 1 pmol/μ L was obtained for all four peptides. Good sensitivity and excellent reproducibility were also achieved. The method expands the application of MALDI MSI from tissues to solutions.

关 键 词：MALDI imaging Quantitative analysis LABEL-FREE MICROARRAY Patterned hydrophilic chip 

分 类 号：O657.63[理学—分析化学] TQ028.8[理学—化学]