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相关领域：医药卫生理学更多>>
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相关机构：内蒙古农业大学首都医科大学附属北京胸科医院哈尔滨商业大学山东中医药大学更多>>
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Label-free and rapid mechanics of single cells under high-density co-culture conditions by deep learning image recognition-assisted atomic force microscopy
《Acta Biochimica et Biophysica Sinica》2025年第2期317-320,共4页Xuliang Yang Mi Li 
supported by the grants from the National Natural Science Foundation of China(No.62273330);the Key Research Program of Frontier Sciences CAS(No.ZDBS-LY-JSC043).
Mechanical cues play an important role in regulating cellular activities.Cells are able to sense and respond to the mechanical cues present in the extracellular physical microenvironment via mechanotransduction[1],whi...
关键词：ORGANS CULTURE dimensions 
施氏鲟精子超低温冷冻前后的蛋白组学分析
《水产学报》2025年第1期25-35,共11页陈张帆 丁兰清 程鹏 胡谋 张婷婷 黄红涛 杜合军 陈松林 
国家重点研发计划(2022YFD2400103);山东省重点研发计划(2021LZGC028,2023ZLYS02);泰山学者攀登计划;中央级公益性科研院所基本科研业务费专项(2023TD20);国家海洋水产种质资源库项目。
【目的】研究施氏鲟精子冷冻损伤分子机理,从遗传水平分析影响施氏鲟精子冷冻复苏后质量的关键因子。【方法】实验采用4D Label-free定量蛋白组学技术筛选施氏鲟精子超低温保存前后的差异蛋白。施氏鲟精子经过24 h冻存后复苏检测,以差...
关键词：施氏鲟 精子冷冻损伤 差异蛋白 4D label-free 
Dark-vacuole Bodies Studying by High-resolution Label-free Microscopy Assisted with Fluorescence Technology
《Chemical Research in Chinese Universities》2024年第6期978-986,共9页LIU Xiangyu ZHANG Jinrui XU Haijiao SHAO Lina WANG Hongda 
supported by the Scientific Instrument Developing Project of the Chinese Academy of Sciences(No.ZDKYYQ20220005);the National Natural Science Foundation of China(Nos.22150003,21727816,and 21721003).
The intracellular logistics system,consisting of vesicles,plays a crucial role in cellular transport.However,there is a lack of research on the types and functions of intracellular vesicles,and new technologies are ne...
关键词：Dark-vacuole body Subcellular Organelle LABEL-FREE Fourier ptychographic microscopy(FPM) Living cell imaging 
Breaking the diffraction limit for label-free chemical imaging
《Advanced Photonics》2024年第6期6-6,共1页Haonan Lin Ji-Xin Cheng 
Super-resolution microscopy has long been a cornerstone in the study of molecular biology,allowing scientists to observe processes at the nanoscale.However,most of these techniques rely on fluorescent labels,which not...
关键词：FIELD LIMIT RESOLUTION 
Label-free Super-resolution Microscopy for Long-term Monitoring the Dynamic Interactions of Cellular Organelles
《Chemical Research in Chinese Universities》2024年第6期994-1003,共10页SHAO Lina SUN Minglu ZHANG Jinrui XU Haijiao YANG Hui LI Hongru WANG Huili CUI Zongqiang LI Dayu WANG Hongda 
supported by the Scientific Instrument Developing Project of the Chinese Academy of Sciences(No.ZDKYYQ20220005);the National Natural Science Foundation of China(Nos.22150003,and 22327808);Chinese patent applications have been submitted for relevant technologies(No.202011538556.8,No.202110126844.0&No.ZL 202120587363.5).
The ideal method for imaging living cells is one that allows for long-term,label-free observation in real-time with super-resolution capabilities.Such a method would overcome the drawbacks of phototoxicity and photobl...
关键词：LABEL-FREE SUPER-RESOLUTION Fourier ptychography microscopy Long-term Cellular organelle SARS-CoV-2 virus-like particle 
Towards next-generation diagnostic pathology:AIempowered label-free multiphoton microscopy
《Light(Science & Applications)》2024年第12期2887-2911,共25页Shu Wang Junlin Pan Xiao Zhang Yueying Li Wenxi Liu Ruolan Lin Xingfu Wang Deyong Kang Zhijun Li Feng Huang Liangyi Chen Jianxin Chen 
support by the National Natural Science Foundation of China(62005049 to S.W.,62072110 to W.L.,82171991 to J.C.,T2288102,32227802,and 81925022,to L.C.);the National Science and Technology Major Project Program(2022YFC3400600 to L.C.);the Natural Science Foundation of Fujian Province(2022J01216 and 2023J011125 to J.C.);supported by New Cornerstone Science Foundation.
Diagnostic pathology,historically dependent on visual scrutiny by experts,is essential for disease detection.Advancesin digital pathology and developments in computer vision technology have led to the application of a...
关键词：MULTIPHOTON refined diagnosis 
Polarization-driven dynamic laser speckle analysis for brain neoplasms differentiation
《Light(Advanced Manufacturing)》2024年第4期31-44,共14页Vahid Abbasian Vahideh Farzam Rad Parisa Shamshiripour Davoud Ahmadvand Arash Darafsheh 
Early diagnosis of brain tumors is often hindered by non-specific symptoms,particularly in eloquent brain regions where open surgery for tissue sampling is unfeasible.This limitation increases the risk of misdiagnosis...
关键词：Polarimetric imaging Dynamic laser speckle analysis Cancerous tissue Brain neoplasm Label-free diagnostics 
Universal MOF nanozyme-induced catalytic amplification strategy for label-free electrochemical immunoassay
《Chinese Chemical Letters》2024年第11期290-293,共4页Ruixin Liu Feng Shi Yanping Xia Haibing Zhu Jiawen Cao Kai Peng Chuanli Ren Juan Li Zhanjun Yang 
financially supported by National Natural Science Foundation of China(Nos.21575125,82172345 and 81573220);the National Natural Science Foundation of Jiangsu Province(No.BK20221370,BK20221281);Key University Natural Science Foundation of Jiangsu-Province(No.20KJA150004);the Project for Science and Technology of Yangzhou(Nos.YZ2022074,YZ2020076);Project for Yangzhou City and Yangzhou University corporation(No.YZ2023204);Cross cooperation project of Subei Peoples’Hospital of Jiangsu Province(No.SBJC220009);Open Research Fund of State Key Laboratory of Analytical Chemistry for Life Science(No.SKLACLS2405);Postgraduate Research&Practice Innovation Program of Jiangsu Province(No.KYCX22_3462)。
Label-free immunoassay is confronted with a great challenge that its insufficient sensitivity for low concentration analytes,which can be assigned to the low catalytic efficiency of modified materials towards electroa...
关键词：MOF nanozyme Catalytic amplification POD-like LABEL-FREE Electrochemical immunosensor 
Label-free in-vivo classi-cation and tracking of red blood cells and platelets using Dynamic-YOLOv4 network被引量：1
《Journal of Innovative Optical Health Sciences》2024年第5期89-103,共15页Caizhong Guan Bin He Hongting Zhang Shangpan Yang Yang Xu Honglian Xiong Yaguang Zeng Mingyi Wang Xunbin Wei 
supported by the National Natural Science Foundation of China(62075042 and 62205060);the Research Fund of Guangdong-Hong Kong-Macao Joint Laboratory for Intelligent Micro-Nano Optoelectronic Technology(2020B1212030010);Fund for Research on National Major Research Instruments of China(Grant No.62027824);Fund for Science and Technology Innovation Cultivation of Guangdong University Students(No.pdjh2022b0543).
In-vivo flow cytometry is a noninvasive real-time diagnostic technique that facilitates continuous monitoring of cells without perturbing their natural biological environment,which renders it a valuable tool for both ...
关键词：LABEL-FREE in-vivoflow cytometry cell classification and tracking D-YOLOv4 
Deep learning-based virtual staining,segmentation,and classification in label-free photoacoustic histology of human specimens
《Light(Science & Applications)》2024年第10期2353-2366,共14页Chiho Yoon Eunwoo Park Sampa Misra Jin Young Kim Jin Woo Baik Kwang Gi Kim Chan Kwon Jung Chulhong Kim 
supported by the following sources:Basic Science Research Program through the National Research Foundation of Korea(NRF)funded by the Ministry of Education(2020R1A6A1A03047902);NRF grant funded by the Ministry of Science and ICT(MSIT)(2023R1A2C3004880,2021M3C1C3097624);Korea Medical Device Development Fund grant funded by the Korea government(MSIT,the Ministry of Trade,Industry and Energy,the Ministry of Health&Welfare,the Ministry of Food and Drug Safety)(Project Number:1711195277,RS-2020-KD000008,1711196475,RS-2023-00243633);Institute of Information&communications Technology Planning&Evaluation(IITP)grant funded by the Korea government(MSIT)(No.RS-2019-II191906,Artificial Intelligence Graduate School Program(POSTECH));BK21 FOUR program.
In pathological diagnostics,histological images highlight the oncological features of excised specimens,but they require laborious and costly staining procedures.Despite recent innovations in label-free microscopy tha...
关键词：STEPWISE Deep SPITE 
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