Universal MOF nanozyme-induced catalytic amplification strategy for label-free electrochemical immunoassay  

作　　者：Ruixin Liu Feng Shi Yanping Xia Haibing Zhu Jiawen Cao Kai Peng Chuanli Ren Juan Li Zhanjun Yang 

机构地区：[1]School of Chemistry and Chemical Engineering,Yangzhou University,Yangzhou 225002,China [2]Clinical Medical College of Yangzhou University,Northern Jiangsu People's Hospital Affiliated to Yangzhou University,Yangzhou University,Yangzhou 225001,China

出　　处：《Chinese Chemical Letters》2024年第11期290-293,共4页中国化学快报（英文版）

基　　金：financially supported by National Natural Science Foundation of China(Nos.21575125,82172345 and 81573220);the National Natural Science Foundation of Jiangsu Province(No.BK20221370,BK20221281);Key University Natural Science Foundation of Jiangsu-Province(No.20KJA150004);the Project for Science and Technology of Yangzhou(Nos.YZ2022074,YZ2020076);Project for Yangzhou City and Yangzhou University corporation(No.YZ2023204);Cross cooperation project of Subei Peoples’Hospital of Jiangsu Province(No.SBJC220009);Open Research Fund of State Key Laboratory of Analytical Chemistry for Life Science(No.SKLACLS2405);Postgraduate Research&Practice Innovation Program of Jiangsu Province(No.KYCX22_3462)。

摘　　要：Label-free immunoassay is confronted with a great challenge that its insufficient sensitivity for low concentration analytes,which can be assigned to the low catalytic efficiency of modified materials towards electroactive molecules.Herein,a universal MOF nanozyme-induced catalytic amplification strategy was proposed for constructing highly sensitive label-free electrochemical immunoassay.Specifically,the synthesized Cu Fe-MOF nanozyme with superior peroxidase(POD)-like activity,regarding as a MOF nanozyme model,can catalyze hydrogen peroxide to produce hydroxyl radicals(·OH),which can efficiently oxidize electroactive probe(such as 1,2-phenylenediamine(o-PD))accompanying with intense electrochemical signals.Modification of MOF nanozyme on the electrode and capture of antibodies for binding target antigens hinder the catalytic process of MOF nanozyme toward o-PD,resulting in a gradual decrease in electrochemical signal with increasing target antigen concentration,enabling quantitative label-free immunoassay.Thus,a highly sensitive label-free immunosensor using MOF nanozyme-induced catalytic amplification achieved effective detection of Immunoglobulin G(Ig G)with a wide linear range of 0.001-50 ng/mL and low detection limit of 0.45 pg/mL.This work proposes a promising nanozyme-induced catalytic amplification strategy for the development of label-free electrochemical immunoassay.

关 键 词：MOF nanozyme Catalytic amplification POD-like LABEL-FREE Electrochemical immunosensor 

分 类 号：O657.1[理学—分析化学] TP212[理学—化学]