Skp2在结直肠癌中的表达及其与VEGF、MVD的关系  

Expression of Skp2 and its Relationship with VEGF and MVD in Colorectal Carcinoma

作　　者：马丹[1] 张丽[2] 徐少勇[3] 

机构地区：[1]武汉大学中南医院消化内科,430071 [2]湖北医药学院附属人民医院病理科 [3]湖北医药学院附属人民医院消化内科

出　　处：《胃肠病学》2016年第6期340-343,共4页Chinese Journal of Gastroenterology

基　　金：湖北省科技厅自然基金资助项目资助(2011CDB127)

摘　　要：背景:近年来结直肠癌的发病率和死亡率呈上升趋势,从基因水平诊断和治疗癌症已成为一种新兴的有效手段。目的:探讨S期激酶相关蛋白2(Skp2)在结直肠癌中的表达及其与血管内皮生长因子(VEGF)、肿瘤微血管密度(MVD)的关系。方法:选取2014年3月—2015年3月湖北医药学院附属人民医院35例结直肠癌患者,以15例癌旁组织作为对照。采用免疫组化SP法检测Skp2、VEGF表达和MVD值,分析其与临床病理特征的关系。结果:结直肠癌组织中Skp2表达显著高于癌旁组织(P=0.000),且与肿瘤分化、淋巴结转移呈正相关(P<0.05),与性别、年龄、TNM分期无明显相关性(P>0.05)。结直肠癌VEGF表达和MVD值显著高于癌旁组织(P=0.019,P=0.002),且与结直肠癌淋巴结转移和TNM分期相关(P<0.05)。结直肠癌Skp2表达与VEGF表达、MVD值均呈正相关(r=0.569,P=0.000;r=0.481,P=0.017)。结论:结直肠癌组织Skp2异常高表达可能与肿瘤血管生成有关,Skp2、VEGF表达和MVD值可作为判断结直肠癌恶性程度的重要指标。Background： In recent years, the incidence and mortality of colorectal carcinoma have been increasing rapidly. Diagnosing and treating cancer at gene level have become a new effective approach. Aims： To investigate the expression of S-phase kinase-associated protein 2 （Skp2） and its relationship with vascular endothelial growth factor （VEGF） and tumor microvessel density （MVD） in colorectal carcinoma. Methods： Thirty-five colorectal carcinoma patients from March 2014 to March 2015 at People＇ s Hospital of Hnbei University of Medicine were enrolled. Fifteen para-cancerous tissue samples were served as controls. The expressions of Skp2, VEGF and MVD value were determined by immunohistochemical SP, and their relationships with clinicopathological characteristics were analyzed. Results ： The expression of Skp2 in colorectal carcinoma was significantly higher than that in para-cancerous tissue （ P = 0. 000 ）, and was correlated with tumor differentiation and lymph node metastasis （ P 〈 0.05 ）, but not with gender, age and TNM stage （ P 〉 0.05 ）. Expression of VEGF and MVD value were significantly higher than those in para-cancerous tissue （P = 0.019, P = 0. 002）, and were correlated with lymph node metastasis and TNM stage （P 〈 0.05 ）. Expression of Skp2 was positively correlated with the expression of VEGF and MVD value in colorectal carcinoma （r = 0. 569, P = 0. 000; r = 0.481, P = 0.017）. Conclusions： The abnormal high expression of Skp2 is involved in the angiogenesis of colorectal carcinoma. Skp2, VEGF expressions and MYD value may be served as imporiant markers for malignancy degree of colorectal carcinoma.

关 键 词：结直肠肿瘤 S期激酶相关蛋白质类 血管内皮生长因子类 微血管密度 

分 类 号：R735.34[医药卫生—肿瘤]