ARNT2在胃癌中的表达及临床意义  

Expression and clinical significance of ARNT2 in gastric carcinoma

作　　者：郝姝桦 郏雁飞[1] 郑燕[1] 马晓丽[1] 孔毅[1] 黎娉[1] 宗帅[1] 张垚[1] 汪运山[1] 

机构地区：[1]山东大学附属济南市中心医院中心实验室,山东济南250013

出　　处：《山东大学学报（医学版）》2016年第7期6-10,共5页Journal of Shandong University:Health Sciences

基　　金：国家自然科学基金(810008869;81272588);国家重点基础研究发展计划(973计划)(2012CB966503;2012CB966504);山东省自然科学基金(ZR2015HM018;ZR2012HM061);山东省优秀中青年科学家科研奖励基金(BS2011YY039)

摘　　要：目的探讨人胃癌中芳香烃受体核易位蛋白2(ARNT2)的表达及临床意义。方法运用qRT-PCR和Western blotting检测ARNT2在正常胃细胞株及多种胃癌细胞株中的表达。通过免疫组织化学检测61例胃癌组织及相应癌旁胃黏膜组织中ARNT2的表达。结果 ARNT2 mRNA和蛋白在胃癌细胞株中的表达水平显著低于正常胃细胞株。胃癌组织中ARNT2的阳性表达率显著低于癌旁胃黏膜组织(32.79%vs 80.33%,P<0.05),与细胞株的检测结果一致。并且,胃癌中ARNT2的表达水平与肿瘤的分化程度显著相关(P<0.05)。结论 ARNT2在胃癌细胞及组织中低表达,并且其表达水平与胃癌的分化程度相关,提示其可能参与胃癌的发生与发展。Objective To investigate the expression of aryl hydrocarbon receptor nuclear translocator 2（ ARNT2） in human gastric carcinoma and its clinical significance. Methods The mRNA and protein expression levels of ARNT2 in normal gastric cell line and several gastric cancer cell lines were measured by quantitative real-time reverse transcriptionpolymerase chain reaction（ qRT-PCR） and Western blotting. The expressions of ARNT2 protein in 61 cases of gastric cancer tissues and adjacent non-tumor tissues were detected by immunohistochemistry. Results The mRNA and protein expressions of ARNT2 in gastric cancer cell lines were significantly lower than those in normal gastric cell line. The positive expression of ARNT2 in gastric cancer tissues was lower than that in adjacent non-tumor tissues（ 32. 79% vs80. 33%,P 〈0. 05）. Furthermore,ARNT2 expression was closely correlated with the differentiation of gastric cancer（ P 〈0. 05）. Conclusion The expression of ARNT2 is lower in gastric cancer cells and tissues than in non-tumor cells and tissues,and is correlated with the differentiation of gastric cancer,which suggests that ARNT2 may participate in the occurrence and development of gastric cancer.

关 键 词：芳香烃受体核易位蛋白2 胃癌 转录因子 

分 类 号：R735.2[医药卫生—肿瘤]