侵染胡椒的黄瓜花叶病毒海南分离物全序列分析及亚组鉴定  

Complete Sequence Analyses and Subgroup Identification of Cucumber Mosaic Virus Hainan Isolates Infecting Piper nigrum L. in Hainan Province

作　　者：刘培培[1,2] 车海彦[2] 曹学仁[2] 杨毅[2] 罗大全[2] 

机构地区：[1]海南大学环境与植物保护学院,海口570228 [2]中国热带农业科学院环境与植物保护研究所,海口571101

出　　处：《热带生物学报》2016年第2期265-269,共5页Journal of Tropical Biology

基　　金：公益性行业(农业)科研专项(项目编号:201303028)

摘　　要：对侵染海南胡椒的3个黄瓜花叶病毒分离物(CMV-WN1,CMV-DA,CMV-FT)的全序进行克隆和分析。CMV-WN1,CMV-DA,CMV-FT分离物RNA1全长分别为3 361,3 360,3 359个核苷酸(nt),编码1a蛋白;RNA2全长分别为3 044,3 048,3 046 nt,编码2a和2b蛋白;RNA3全长分别为2 217,2 224,2 216 nt,编码3a和CP蛋白。序列一致性比较结果表明,CMV-WN1,CMV-DA,CMV-FT的RNA1,RNA2,RNA3均与CMV亚组IB CMV-SD序列一致性最高,编码的所有蛋白的氨基酸序列一致性也均与CMV-SD序列一致性最高,其中除了2b氨基酸序列一致性低于90%外,其他蛋白的氨基酸序列的一致性均高于93.5%。RNA3的5'NTR结构分析以及RNA3 5'NTR核苷酸序列和CP氨基酸序列系统进化树分析结果表明CMV-WN1,CMV-DA及CMV-FT均属CMV IB亚组。CP系统进化树中CMV-WN1,CMV-FT与云南胡椒分离物CMV-YNP聚成一簇,而CMV-DA与海南胡椒分离物CMV-HNP独立形成另一个分支。The full-length c DNA sequences of genomic RNAs of CMV-WN1,DA and FT isolates from the leaves of black pepper（ Piper nigrum L.） infected with cucumber mosaic virus in Hainan Province were determined by using RT-PCR and sequence analysis. The RNA1 of the CMV-WN1,DA and FT consisted of 3361,3360 and3359 nucleotides（ nt）,respectively,and encoded protein 1a. The RNA2 consisted of 3044,3048 and 3046 nt,respectively,and encoded protein 2a and 2b. The RNA3 contained 2217,2224 and 2216 nt,respectively,and encoded protein 3a and CP. The CMV-WN1,DA and FT isolates had the highest similarity in the sequences of RNA 1,RNA2 and RNA3 with the CMV-SD strain,and their amino acid sequences of all encoding proteins were also most highly similar with that of the CMV-Strain,of which the amino acid sequence of all the proteins had a93. 5% similarity with that of the CMV-SD except that 2b amino acid sequence shared a similarity of 90% or less. Analysis of the 5＇NTR structure of RNA3,the phylogenetic tree analyses of 5＇NTR nucleotide sequences of RNA3 and CP amino acid sequences showed that the CMV-WN1,DA and FT isolates were classified into CMV IB subgroup. The phylogenetic tree analyses of CP amino acid sequences also showed that CMV-WN1,CMV-FT and the isolate of the black pepper in Yunnan,CMV-YNP,were clustered into the same group while the CMVDA and the isolate of the black pepper in Hainan,CMV-HNP,were clustered into another separate group.

关 键 词：胡椒 黄瓜花叶病毒 海南分离物 核酸 全序列分析 

分 类 号：S432.1[农业科学—植物病理学]