大孔树脂纯化后益智总黄酮体外抗氧化活性研究  

The research of the in vitro antioxidant ability of purified flavonoids from Alpiniae Oxyphyllae Fructus

作　　者：刘春梅[1] 吴博[1] 刘冰[1] 赵旭[1] 毕开顺[2] 贾英[1] 

机构地区：[1]沈阳药科大学中药学院,沈阳市中药药效物质基础筛选与评价重点实验室,辽宁沈阳110016 [2]沈阳药科大学药学院,中药质量控制关键技术国家地方联合工程实验室,辽宁沈阳110016

出　　处：《时珍国医国药》2016年第6期1321-1324,共4页Lishizhen Medicine and Materia Medica Research

基　　金：国家自然科学基金(No.81403065);辽宁省自然科学基金(No.2014020076);辽宁省沈阳市科技计划项目(No.F13-287-1-00)

摘　　要：目的研究大孔树脂纯化得到的益智总黄酮的体外抗氧化活性。方法以大孔树脂纯化得到的益智总黄酮为研究对象,并以Vc为对照,对其还原能力及1,1-二苯基-2-苦苯肼(·DPPH)自由基、·ABTS+自由基、羟基(·OH)自由基、超氧阴离子(O_2-·)自由基的清除能力和对铁氰化钾还原能力进行探讨。结果纯化后黄酮含量为51.68%的益智总黄酮对·DPPH自由基、·ABTS+自由基、羟基(·OH)自由基、超氧阴离子(O_2-·)自由基的清除能力的IC50分别为0.122 mg/ml、0.656 mg/ml、2.571 mg/ml和1.458 mg/ml,且对铁氰化钾还原能力的EC50为0.064 mg/m L,因此具有较强的还原能力。结论益智总黄酮表现出良好的体外抗氧化活性,为益智今后的研究提供了理论依据。Objective To analyze the in vitro antioxidant capacities of flavonoids from Alpiniae Oxyphyllae Fructus. Methods The flavonoids was evaluated by five models of antioxidant activity： ·DPPH,ABTS＋·,·OH,O_2-·,Fe^（3＋）,with Vc as reference substance. Results The results showed that compared with the positive control Vc,the flavonoids from Alpiniae oxyphyllae Fructus had stronger capacity of scavenging on ·DPPH,ABTS＋·,·OH,O_2-· with IC50 of 0. 122 mg / m L,0. 656 mg / m L,2. 571 mg / m L and 1. 458 mg / m L,respectively,and strong reduction capacity of Fe^（3＋）. It was indicated that the flavonoids from A.oxyphyllae Fructus had certain antioxidant capacity. Conclusion The purified flavonoids from A. oxyphyllae Fructus exhibit obviously antioxidation ability,and also showed a higher reduction capability. This provided the basis for the further development of A. oxyphyllae Fructus.

关 键 词：益智 黄酮 自由基 体外抗氧化 

分 类 号：R285.5[医药卫生—中药学]