不同产地杜仲叶高效液相色谱指纹图谱分析  

HPLC fingerprint of Eucommiae Folium from different habitats

作　　者：唐芳瑞[1] 刘荣华[1] 邵峰[1] 李于益 张忠立[1] 黄慧莲[1] 

机构地区：[1]江西中医药大学,江西南昌330004

出　　处：《时珍国医国药》2016年第6期1496-1498,共3页Lishizhen Medicine and Materia Medica Research

基　　金：国家自然科学基金资助项目(No.81160508)

摘　　要：目的建立杜仲叶HPLC指纹图谱分析方法,为杜仲叶的质量评价提供参考。方法色谱柱:YMC-Triact C_(18)(250mm×4.6 mm,5.0μm);流动相为乙腈-0.1%H_3PO_4,梯度洗脱;流速为1.0 ml·min^(-1);检测波长208 nm;柱温30℃,测定17个产地的杜仲叶指纹图谱,采用《中药色谱指纹图谱相似度评价系统(2004A版)》软件和SPSS软件对其进行相似度和系统聚类分析。结果确定了杜仲叶HPLC指纹图谱中的30个共有指纹峰,对17批杜仲叶指纹图谱进行相似度分析,相似度在0.828~0.979之间,聚类分析结果为3大类,第I类(S10)为四川省达州市渠县所产,第II类(S3、S2、S7)分别为江西省南昌市湾里区、江西省赣州市崇义县、湖南省张家界市永定区所产,其余为第III类。结论该研究方法准确、可靠、重现性好,可为杜仲叶药材质量评价提供依据。Objective To establish the fingerprints of Eucommiae Folium from different habitats by HPLC. Methods The chromatographic fingerprint was obtained with YMC- Triact C_（18） column（ 250 mm × 4. 6 mm,5. 0 μm） and gradient eluted with acetonitrile and water（ with 0. 1% phosphoric acid）. The column temperature was maintained at 30 ℃,and the flow rate was 1 ml·min^（- 1）. The detection wavelength was 208 nm. The injection volume was 20 μl. Results A total of main 30 common characteristic peaks were found,the mutual mode of HPLC fingerprints was set up and the similar degrees to the crude drugs from different habitats were compared by similarity evaluation system for chromatographic fingerprint of CMM（ Version 2004A）,and the range of similarity for 17 balches of Eucommiae Folium 0. 828 ~ 0. 979. 17 balches of Eucommiae Folium were divided into three groups based on the result of hierarchical clustering analysis. Conclusion This method is accurate and reliable,and provides a scientific basis for the quality control of Eucommiae Folium.

关 键 词：杜仲叶 高效液相色谱 指纹图谱 相似度 聚类分析 

分 类 号：R284.2[医药卫生—中药学]