唾液酸酶对子宫内膜癌细胞增殖和凋亡的影响  

Effects of Sialidase Inhibitor on Proliferation and Apoptosis of Endometrial Cancer Cells

作　　者：熊英[1] 张建国[2] 苏冬梅[3] 莫家明[2] 李思[2] 马芳[1,4] 

机构地区：[1]四川大学华西第二医院妇产科,成都610041 [2]四川大学华西基础医学与法医学院形态学教研室,成都610041 [3]四川大学华西临床医学院病理研究室,成都610041 [4]四川大学华西第二医院四川大学-香港中文大学生殖医学联合实验室,成都610041

出　　处：《四川大学学报（医学版）》2016年第4期458-462,467,共6页Journal of Sichuan University(Medical Sciences)

基　　金：国家自然科学基金(No.31470797)资助

摘　　要：目的探讨唾液酸酶与子宫内膜癌细胞Ishikawa浸润、增殖和凋亡的关系。方法培养子宫内膜癌Ishikawa细胞,并分成对照组和处理组,处理组给予唾液酸酶活性抑制剂(DANA)处理,比对两组细胞上清液的唾液酸酶活性后通过免疫荧光检测、流式细胞学或MTT实验检测对照组和处理组细胞基质金属蛋白酶(MMPs)表达水平、细胞增殖核抗原(PCNA)水平、凋亡和增殖情况。结果处理组上清液唾液酸酶活性减弱且细胞MMPs的表达降低,处理组的细胞凋亡率明显降低,各组细胞的增殖能力无差异。结论唾液酸酶可影响Ishikawa细胞的侵袭性和凋亡率,但对细胞的增殖能力无明显影响。Objective To explore the effects of sialidase inhibitor on the proliferation and apoptosis of endometrial cancer Ishikawa cells.Methods Ishikawa cells were cultured and divided into 2groups：control group and sialidase inhibitor N-Acetyl-2,3-dehydro-2-deoxyneuraminic acid（DANA）treated group.After the sialidase activity was compared between the two groups,the expression level of matrix metalloproteinases（MMPs）,proliferating cell nuclear antigen（PCNA）, apoptosis rate and proliferation ability were detected by immunofluorescence staining,flow cytometry or MTT assay.Results With the treatment of DANA,the activity of sialidase in the cell culture supernatant was suppressed while MMPs expression levels and apoptosis rate of Ishikawa cells were decreased.The expression level of PCNA and cell proliferation showed no statistical differences in two groups.Conclusion Sialidase could affect the invasive ability and apoptosis rate of Ishikawa cells,but it seems no effect on cell proliferation.

关 键 词：唾液酸酶 肿瘤 增殖 凋亡 

分 类 号：R737.33[医药卫生—肿瘤]