蔗渣纤维素分解菌的筛选、鉴定及酶活的测定  

Screening, Identification and Enzyme Activity Assay of Bagasse Celluloytic Microbes

作　　者：黄惠娟[1] 方界群 陈华文[2] 陈迪文[1] 黄莹[1] 黄振瑞[1] 江永[1] 

机构地区：[1]广州甘蔗糖业研究所广东省甘蔗改良与生物炼制重点实验室,广东广州510316 [2]广东省遂溪县农业局,广东遂溪524300

出　　处：《甘蔗糖业》2016年第3期42-45,共4页Sugarcane and Canesugar

基　　金：南方高蓄能作物养分资源综合管理创新能力建设(2015B070701001);南方高秆作物农用化学品减量化关键技术创新能力建设(2014B070706005);现代甘蔗育种创新能力建设(2014B070705002);国家现代农业产业技术体系甘蔗专项(CARS-20-3-1)

摘　　要：为充分利用蔗渣,筛选高效降解蔗渣的菌株,本文从堆肥中分离得到有显著透明圈、可产纤维素酶的真菌8株,选其中3株测定了羧甲基纤维素酶(CMCase)和滤纸酶(FPA)活性。结果表明,菌株SC8的透明圈直径、CMCase和FPA活性均最大。经菌落形态特征分析,初步鉴定菌株SC8属于曲霉属(Aspergillus)。相关性分析表明,所筛选菌株在纤维素透明圈直径的大小与CMCase、FPA酶活性成极显著正相关(p<0.01)。In order to make full use of bagasse, this study was conducted to screen high efficient bagasse degradation microorganisms fi＇om compost. 8 strains of fungi with significant transparent circles and cellulase activity were screened and 3 of them were selected to determine the activity of carboxymethyl cellulase （CMCase） and filter paper activity （FPA）. The results showed that the size of transparent circle and the activity of CMCase and FPA of SC8 were the highest among the 8 screened strains. Based on morphology analysis, the fungus SC8 was initially classified into the genus of Aspergillus. Correlation analysis showed that the size of transparent circle was in sit, nificant positive correlation with the activity of CMCase and FPA （p〈0.0 1）.

关 键 词：蔗渣 纤维素分解菌 纤维素酶 滤纸酶活 

分 类 号：S566.1[农业科学—作物学]