银线草HPLC指纹图谱研究  

HPLC Fingerprint of Chloranthus japonicus Sieb.

作　　者：袁琴琴[1,2] 

机构地区：[1]菏泽学院生命科学系,山东菏泽274000 [2]陕西师范大学药用资源与天然药物化学教育部重点实验室,西北濒危药材资源开发国家工程实验室,陕西西安710062

出　　处：《安徽农业科学》2016年第13期138-140,160,共4页Journal of Anhui Agricultural Sciences

基　　金：西安市科技创新支撑计划项目[CXY1018(3)];中央高校基本科研业务费专项资金项目(CK201002010)

摘　　要：[目的]建立银线草HPLC指纹图谱研究方法,为其质量控制提供依据。[方法]采用高效液相色谱法,色谱柱为Phenomenex C_（18）（250.0 mm×4.6 mm,5.0μm）;流动相甲醇-0.1%甲酸线性梯度洗脱,流速为0.8 m L/min;检测波长323 nm;柱温25℃。[结果]建立了银线草HPLC指纹图谱共有模式,并标定12个共有峰,13批样品指纹图谱相似度在0.714~0.957。[结论]不同产地的银线草化学成分存在一定差异,所建立的指纹图谱方法简单、准确可靠、重复性好,为银线草质量标准的制定提供科学依据。[Objective]To establish a method of HPLC fingerprint analysis on Chloranthus japonicus Sieb.,and to provide references for the quality control. [Method] High-performance liquid chromatography was adopted. The chromatographic column was Phenomenex C_（18） （ 250. 0 mm × 4. 6 mm,5. 0 μm）; mobile phase was methanol-0. 1% formic acid with linear gradient elution; flow rate was 0. 8 m L/min;detection wavelength was 323 nm and the column temperature was maintained at 25 ℃. [Result]HPLC fingerprint of C. japonicus was established. Twelve main marker peaks were selected. Fingerprint similarities of 13 sample batches were distributed between 0. 714 and 0. 957.[Conclusion]There are certain differences in the chemical components of C. japonicus in different production areas. The established fingerprint method is simple,accurate,reliable and repeatable,which provides scientific references for the quality standard establishment of C. japonicus.

关 键 词：银线草 HPLC 指纹图谱 

分 类 号：S188[农业科学—农业基础科学]