赖氨酰氧化酶样蛋白2及E-钙黏蛋白在三阴性乳腺癌中的表达及临床意义  

Expressions of lysyl oxidase-like protein 2 and E-cadherin in triple-negative breast cancer and clinical significance

作　　者：孙吉瑞 张金库[1] 周炳娟[1] 乔海芝 陈红[1] 马秋双[1] 陈雪[1] 

机构地区：[1]保定市第一中心医院病理科,071000

出　　处：《中华乳腺病杂志（电子版）》2016年第3期151-154,共4页Chinese Journal of Breast Disease(Electronic Edition)

摘　　要：目的探讨赖氨酰氧化酶样蛋白2(LOXL2)、E-钙黏蛋白(E-cadherin)在三阴性乳腺癌(TNBC)中的表达及相互关系。方法收集2008年8月至2014年3月在保定市第一中心医院治疗的浸润性乳腺癌患者的组织标本进行回顾性分析,包括TNBC 66例及其癌旁正常乳腺组织,非TNBC(non-TNBC)70例。采用免疫组织化学方法检测3组中LOXL2及E-cadherin的表达。LOXL2和E-cadherin的表达及其与TNBC患者临床病理因素的关系采用χ2检验,LOXL2和E-cadherin相关性分析采用Spearman等级相关性分析。结果 LOXL2在TNBC组、non-TNBC组和正常乳腺组织中的阳性表达率分别为74.2%(49/66)、51.4%(36/70)和21.2%(14/66),差异有统计学意义(χ2=37.387,P<0.001)。TNBC组的LOXL2阳性表达率高于non-TNBC组及正常乳腺组织(χ2=7.544、37.198,P均<0.017)。E-cadherin在TNBC、non-TNBC及癌旁正常乳腺组织中的阳性表达率分别为24.2%(16/66)、41.4%(29/70)和97.0%(64/66),差异有统计学意义(χ2=77.031,P<0.001)。TNBC组的E-cadherin阳性表达率低于正常乳腺组织(χ2=73.108,P<0.017),但是与non-TNBC组比较,差异无统计学意义(χ2=4.532,P>0.017)。在TNBC患者中,LOXL2和E-cadherin的表达与淋巴结转移(χ2=11.509、9.964,P=0.001、0.003)及临床分期(χ2=7.436、9.175,P=0.006、0.002)有关,与患者的年龄(χ2=0.169、0.539,P=0.681、0.463)和肿瘤直径(χ2=1.418、0.592,P=0.492、0.744)无关。LOXL2与E-cadherin蛋白在TNBC中的表达呈负相关(r=-0.475,P<0.001)。结论 LOXL2可能成为新的判断TNBC预后的分子标志物。Objective To investigate the expressions of lysyl oxidase-like protein 2 （LOXL2） and E-cadherin in triple-negative breast cancer （TNBC） and their correlation. Methods The tissue samples from invasive breast cancer patients treated in First Central Hospital of Baoding City from August 2008 to March 2014 were collected for retrospective analysis, including 66 cases of TNBC and their normal breast tissue as control, 70 cases of non-TNBC. Immunohistochemistry was used to detect the expressions of LOXL2 and E-cadherin in samples. The expressions of LOXL2 and E-cadherin and their relationship with clinicopathological parameters were compared using χ^2 test, and the correlation of LOXL2 and E-cadherin was analyzed using Spearman correlation analysis. Results The positive rate of LOXL2 was 74.2% （49/66） in TNBC group, 51.4% （36/70） in non-TNBC group and 21.2% （14/66） in control group, which indicated a significant difference （χ^2= 37. 387 ,P〈0. 001 ）. LOXL2 positive rate in TNBC group was significantly higher than that in non-TNBC group or in normal breast tissue （χ^2 = 7. 544, 37. 198, both P〈0. 017）. The positive rate of E-cadherin was 24. 2% （16/66） in TNBC group, 41.4% （29/70） in non-TNBC group and 97.0% （64/66） in control group, which indicated a significant difference （χ^2= 77. 031, P〈0. 001 ）. E-cadherin positive rate in TNBC group was significantly lower than that in normal breast tissue （ χ^2 = 73. 108, P 〈 0. 017）, but there was no significant difference between TNBC group and non-TNBC group （ χ^2 = 4. 532, P〉 0. 017）. In TNBC patients, the expressions of LOXL2 and E-cadherin were correlated with lymph nodemetastasis （χ^2 = 11. 509, 9. 964 ; P = 0. 001, 0. 003 ） and TNM staging （χ^2 = 7. 436, 9. 175 ; P = 0. 006, 0. 002）, but they were not correlated with the patients＇age （χ^2=0. 169, 0. 539 ; P= 0. 681, 0. 463 ） and tumor diameter 0（2 = 1. 418, 0. 592; P = 0. 492, 0. 744）. LOXL2 was negatively correlated with E-eadherin in

关 键 词：乳腺肿瘤 受体 ERBB-2 钙粘着糖蛋白类 

分 类 号：R737.9[医药卫生—肿瘤]