异种源性脱细胞神经支架修复坐骨神经损伤后再生的可行性  

The Feasibility of Xenogeneic Acellular Nerve Scaffold to Repair Rat Sciatic Nerve Defect Regeneration

作　　者：陈少红[1] 季婉青 侯博[3] 

机构地区：[1]广东省人民政府机关门诊部,广东广州510030 [2]广州市妇女儿童医疗中心 [3]中山大学附属第三医院

出　　处：《中国医学创新》2016年第20期27-32,共6页Medical Innovation of China

基　　金：广东省自然科学基金(2014A030313189;2014A030310408);广东省科技计划项目(2014A020211013;2015A020212016;2016A020214007)

摘　　要：目的:评价异种脱细胞神经支架修复大鼠坐骨神经损伤的疗效。方法:以兔源性胫神经为原材料,通过萃取技术制备脱细胞神经支架;HE染色、甲苯胺蓝染色、免疫组化(IHC)、扫描电镜(SEM)、透射电镜(TEM)检测技术评价脱细胞效果;体内实验部分采用坐骨神经离断模型,按照移植物的不同,将SD大鼠随机分为两组:异种神经支架移植组(实验组)、自体神经移植组(对照组),术后12周内,流式细胞术检测大鼠外周血中CD3^+、CD4^+、CD8^+淋巴细胞含量,以判定机体免疫系统情况;通过神经功能测定及形态学手段评价10 mm缺损神经的修复效果。结果:支架内的细胞和髓鞘成分能够彻底去除,基底膜管状结构保留较为完整。术后1、4、12周时间点,实验组大鼠外周血CD3^+、CD4^+、CD8^+淋巴细胞含量与对照组比较,差异均无统计学意义(P>0.05)。4周及12周后,两组坐骨神经功能指数(SFI)比较差异均无统计学意义(P>0.05);实验组移植物内的再生轴突数量、再生髓鞘厚度方面与对照组比较,差异均无统计学意义(P>0.05)。结论:异种神经脱细胞支架干预10 mm大鼠坐骨神经缺损,并不会引起机体系统性排斥反应,同时能够有效促进损伤神经再生。Objective：To evaluate remodeling effect of xenogeneic acellular nerve scaffold in a rat sciatic nerve defect model.Method：Scaffolds derived from rabbit tibial nerves were processed by extractive method,and results were monitored by HE,toluidine blue,immunohistochemistry, SEM,TEM.SD rats were randomly divided into two groups：xenogeneic acellular nerve scaffold group（experimental group） and autograft （control group） group.During 12 weeks postoperatively,the amount of CD3^＋,CD4^＋,CD8^＋ in blood were measured to evaluate the systematic immunologic function.Regenerative results were determined by SFI test,and histological examination. Result：Cell component and myelin sheath were completely removed and intact basal lumina tubes were persistent. During observation,all animals had no sign of immunological rejection systematically,grafts bridged well without any disintegration.In the experimental group,the amount of CD3＋,CD4＋,CD8＋ in blood were not different with the control groups at 1,4,12 weeks postoperatively（P〉0.05）.Statistical analysis revealed that experimental group had no statistically significant differences between the experimental group and control group on aspect of SFI at 4,12 weeks（P〉0.05）.There had no statistically significant differences between the experimental group and control group on axon number and myelin sheath depth（P〉0.05）.Conclusion：Xenogeneic acellular nerve scaffold may be a promising approach in repairing peripheral nerve defect.

关 键 词：异种 细胞外基质 支架 神经再生 免疫排斥反应 

分 类 号：R745.4[医药卫生—神经病学与精神病学]