血管扩张刺激磷蛋白的表达与前列腺癌转移的关系  

Expression of vasodilator-stimulated phosphoprotein relates to prostate cancer metastasis

作　　者：李中兴[1] 张德玲[2] 葛广成[1] 王星[1] 吴丹[1] 贾跃军[1] 冯瑞[1] 

机构地区：[1]镇江市第二人民医院泌尿外科,江苏镇江212002 [2]武汉大学基础医学院病理生理学教研室,湖北武汉430071

出　　处：《中华男科学杂志》2016年第7期608-612,共5页National Journal of Andrology

基　　金：镇江市科学技术局社会发展项目(SH2012045)~~

摘　　要：目的:研究血管扩张刺激磷蛋白(VASP)与前列腺癌侵袭转移及预后的关系。方法:将VASP shRNA慢病毒和对照shRNA慢病毒分别感染前列腺癌PC3细胞,采用跨膜迁移实验检测PC3细胞的侵袭能力;采用免疫组化法检测56例前列腺癌患者癌组织中VASP的表达,并根据VASP表达差异及患者前列腺癌根治术后随访结果进行生存分析比较。结果:与shRNA慢病毒对照组及空白对照组相比,VASP shRNA慢病毒可抑制前列腺癌PC3细胞VASP的表达,并且显著降低PC3细胞的侵袭能力(P<0.05);对56例前列腺癌患者的生存分析表明,与VASP阴性表达组相比,VASP阳性表达组及VASP强阳性表达组患者生化复发时间显著缩短(P<0.05),后两者之间比较差异无统计学意义(P>0.05)。结论:VASP参与调控前列腺癌PC3细胞的侵袭能力;VASP蛋白表达差异与前列腺癌患者的预后相关。Objective： To investigate the relationship of the expression of vasodilator-stimulated phosphoprotein （VASP） with the metastasis and prognosis of prostate cancer. Methods： Prostate cancer PC3 cells were infected with VASP shRNA and control shRNA lentiviruses, respectively. The invasive ability of the PC3 cells was determined by transwell migration assay, the expression of VASP in the prostate cancer tissue from 56 patients was detected by immunohistochemistry, and the survival rate of the patients was an- alyzed according to the VASP expression levels and follow-up data after radical prostatectomy. Results： VASP shRNA lentivirus sig- nificantly inhibited the expression of VASP and decreased the invasive ability of the PC3 cells as compared with the results obtained in the scramble shRNA and blank control groups （ P 〈 0.05 ）. The survival analysis of the 56 prostate cancer patients showed that the time of biochemical recurrence was markedly shorter in the VASP positive and strongly positive groups than in the VASP-negative cases （P 〈 0.05 ）, but with no statistically significant difference between the former two groups （ P 〉 0.05 ）. Conclusion ： VASP is in- volved in the regulation of the invasive ability of prostate cancer PC3 cells, and the differences in the VASP expression are related to the prognosis of prostate cancer.

关 键 词：前列腺癌 血管扩张刺激磷蛋白 PC3细胞 肿瘤转移 

分 类 号：R737.25[医药卫生—肿瘤]