Tbx18^+心外膜祖细胞参与成年小鼠部分心脏组织形成  

Tbx18^+ Epicardial Progenitor Cells Contributed to the Formation of Partial Cardiac Tissue of Adult Mice

作　　者：杜建霖[1] 邓松柏[1] 刘亚杰[1] 佘强[1] 

机构地区：[1]重庆医科大学附属第二医院心血管内科,重庆400010

出　　处：《中国生物化学与分子生物学报》2016年第7期837-840,共4页Chinese Journal of Biochemistry and Molecular Biology

基　　金：国家自然科学基金(No.81270211);重庆教委科学技术研究项目(No.KJ130324);重庆市科委前沿与应用基础研究计划项目(No.cstc2015jcyj A10008;No.cstc2015jcyj A10070)资助~~

摘　　要：转录因子Tbx18在胚胎心脏发育过程中起重要调控作用,是心外膜祖细胞标记之一;故以Tbx18为标记的阳性祖细胞群被称为:Tbx18+心外膜祖细胞(epicardial progenitor cells,EPCs)。小鼠胚胎、新生和成年期心脏组织细胞的特性区别较大,成年小鼠的心脏属于终末分化组织。但是,Tbx18+EPCs对成年小鼠心脏组织的贡献大小尚存争议。本研究拟定量分析Tbx18+EPCs对成年小鼠心脏组织的贡献大小。采用整体和组织切片X-gal染色检测成年心脏组织Lac Z的表达;荧光激活细胞分选法(fluorescence activated cell sorting,FACS)分离成年Tbx18Cre/R26EYFP小鼠心脏组织EYFP+细胞。结果显示,在Tbx18+EPCs遗传谱系示踪小鼠,报告基因Lac Z和EYFP在成年小鼠心脏的心室、心房、冠状动脉、室间隔等处表达;成年Tbx18Cre/R26EYFP小鼠心脏组织细胞用FACS分离,分选的EYFP+细胞比例平均约为33.94%。由此可见,成年小鼠心脏的心室、心房、冠状动脉、室间隔等心脏组织均可来源于Tbx18+EPCs;约1/3成年小鼠心脏组织细胞来源于Tbx18+EPCs。故Tbx18+EPCs参与成年小鼠心脏组织的部分形成。Transcription factor Tbx18 plays a vital role in controlling the development of embryonic heart,and it is one of the markers of epicardial progenitor cells;therefore,those positive progenitor cell groups marked with Tbx18 are named as ＂Tbx18＋EPCs（epicardial progenitor cells） ＂.The embryonic,new born and adult mice＇s cardiac histocytes differed a lot in their characteristics,and the heart of an adult mouse is already ultimate differentiated tissue.However,researchers are still debating about the role of Tbx18＋EPCs in mice＇s cardiac development.In this study,we tried to do a quantitative analysis of its role in adult mice＇s cardiac development.We tried to examine the Lac Z expression in adult cardiac tissue through observing whole mount and tissue sections ＇ X-gal staining method,and by using fluorescence activated cell sorting（FACS） method to sort out Tbx18 Cre/R26EYFPmouse＇s EYFP＋cells in heart.The results showed that on Tbx18＋EPCs-genetic-lineage-tracing-mouse,reporter gene EYFP and Lac Z were expressed in ventricles,atria,coronary artery,interventricular septum in their adults＇ heart;the cardiac cells are isolated by FACS in adult Tbx18 Cre/R26 EYFPmouse,the positivity ratio of EYFP＋cells is 33.94% in average.Thus,the histiocyte of adult mouse＇s ventricle,atrium,coronary artery,interventricular septum can be derived from Tbx18＋EPCs;about 1/3 of adult mouse＇s cardiac histocytes may originate from Tbx18＋EPCs.Therefore Tbx18＋EPCs partially involved in the formation of the cardiac tissue of adult mice.

关 键 词：T-box转录因子18 心脏祖细胞 遗传谱系示踪 成年 

分 类 号：R394.1[医药卫生—医学遗传学]