1株与大肠埃希菌O157生化结果相似的志贺氏菌的鉴定  

Identification of a strain of Shigella with biochemical features similar to Escherichia coli O157

作　　者：阚式绂 黄裕 王明明 

机构地区：[1]深圳市农产品质量安全检验检测中心,深圳518005 [2]深圳华大基因研究院,深圳518083

出　　处：《食品安全质量检测学报》2016年第6期2258-2262,共5页Journal of Food Safety and Quality

摘　　要：目的对一菌株sznjO23进行分析鉴定。方法从猪肝中分离到菌株sznjO23,并对该菌株的菌落形态、生化特征及分子生物学特性进行分析。结果该菌株在CT-SMAC平板上呈圆形、光滑、较小的无色菌落,在改良CHROMagarO157显色平板上为紫色圆形的菌落。经全自动微生物鉴定分析系统VITEK 2 Compact鉴定,其生化结果96%可能性为大肠埃希菌O157,但血清学试验结果反应完全混浊,未出现任何凝集。16SrDNA序列分析发现,该菌株与志贺氏菌(ATCC29903)的进化距离较近、同源性高,相似度为99.26%。结论菌株sznjO23为志贺氏菌。当生化试验、血清学试验结果不一致时可选择16SrDNA序列分析的方法进行鉴定,以保证结果的准确性。Objective To analyze and identify a bacterium of sznjO23. Methods A strain of sznjO23 was isolated from pig＇s liver, and the colonial morphology, biochemical features, and molecular biological characteristics of the strain were analyzed. Results Its colonies features on CT-SMAC were rounded, smooth, small, and colorless, while the strain became purple and circular on CHROMagar O157. Biochemical results showed that the strain had 96% possibility to be E. coli O157 by VITEK 2 Compact. Serology reaction was cloudy, and it didn＇t appear any agglutination. The sequence analysis result of 16S rDNA showed that the strain was closed to Shigella（ATCC29903） from evolutionary distance with the similarity of 99.26%. Conclusion The strain of sznjO23 was Shigella. When the results of biochemical tests and serological test were inconsistent, 16S rDNA sequence analysis can be used in order to ensure the accuracy of the results.

关 键 词：志贺氏菌 生化试验 血清学试验 16SRDNA序列 

分 类 号：R446.5[医药卫生—诊断学]