神经干细胞玻璃体腔移植对糖尿病大鼠视网膜功能及结构的影响  

The effects on the function and structure of retina in diabetic rats by intravitreai transplantation of human umbilical cord cells-induced neuronal stem cells

作　　者：姜鉴洪 孔佳慧[1] 陈松[1] 段红涛[1] 王月欣[1] 董蒙[1] 张惟[1] 王昀[1] 林锦镛[1] 

机构地区：[1]天津医科大学眼科临床学院天津市眼科医院天津市眼科学与视觉科学重点实验室天津市眼科研究所,300020

出　　处：《中华眼底病杂志》2016年第4期418-422,共5页Chinese Journal of Ocular Fundus Diseases

基　　金：基金项目：天津市科技计划项目（13ZCZDSY01500）

摘　　要：目的 观察玻璃体腔注射人脐带间充质干细胞（hUCMSC）体外诱导的神经干细胞（NSC）对糖尿病大鼠视网膜功能及结构的影响。方法 健康雄性Sprague-Dawley大鼠40只，随机分为正常对照组（A组）、糖尿病组，分别为9、31只。糖尿病组大鼠经腹腔注射链脲佐菌素诱导糖尿病模型。成模10周时，将建模成功的27只大鼠随机分为糖尿病对照组（B组）、玻璃体腔注射磷酸盐缓冲液组（C组）、玻璃体腔注射NSC组（D组），各组均为9只大鼠。A、B组不进行干预。干预后2、4、6周A^D组大鼠均行闪光视网膜电图检查，暗适应条件下记录暗适应视杆细胞反应（Rod-R）b波潜伏期和振幅以及最大混合反应（Max-R）a、b波潜伏期和振幅、振荡电位（OPs）总振幅。苏木精-伊红染色观察各组大鼠视网膜形态结构变化。结果 干预后2、4周，A～D组大鼠暗适应Rod-R b波振幅、Max-R a、b波潜伏期及振幅、OPs总振幅比较，差异均有统计学意义（P＜0.05）；D组暗适应Rod-R b波振幅、Max-R b波振幅、OPs总振幅较B、C组提高，差异均有统计学意义（P＜0.05）；Max-R b波潜伏期较B、C组下降，差异有统计学意义（P＜0.05）。干预后6周，D组暗适应Rod-R b波振幅、Max-R a、b波振幅、OPs总振幅较B、C组提高，差异有统计学意义（P＜0.05）；D组Rod-R、Max-R b波潜伏期较C组下降，差异有统计学意义（P＜0.05）。建模后10周，与A组比较，糖尿病组大鼠视网膜各层细胞排列紊乱。干预后2周，与A组比较，B、C组视网膜各层细胞排列紊乱，内界膜连续性破坏，局部增厚；4周，D组视网膜各层细胞排列紊乱，视网膜神经节细胞数量较B、C组增多，血管扩张不明显。结论 玻璃体腔注射hUCMSC体外诱导的NSC对糖尿病大鼠视网膜功能及结构均有改善作用。Objective To observe the effects on the function and structure of retina in diabetic rats by intravitreal transplantation of retinal nerve stem cells （NSC） differentiated from human umbilical cord mesenchymal stem cells （hUCMSCs）. Methods Fifty clean male Sprague-Dawley rats were randomly divided into normal control with 9 rats （group A） and diabetes mellitus （DM） group with 31 rats. The DM models were induced by intraperitoneal injection of streptozocin. The rats of DM group were randomly divided into four groups after 10 weeks： rats with DM only （group B）, diabetic rats with saline intravitreal injection （group C）, diabetic rats with NSC intravitreal injection （group D）, and 9 rats for each. The rats in the group A and B received no treatment. The retinal function was examined by the flash-electroretinogram on 2, 4, 6 weeks after intervention, the latency and amplitude of a-wave, b-wave of Rod, a-wave, b-wave of Max reactions （Max-R） and the total amplitudes of OPs were recorded. The morphological changes of retina were observed by hematoxylin-eosin staining. Results On 2 and 4 weeks after the intervention, the differences of latency and amplitude of b-wave of Rod, a-wave, b-wave of Max-R and the total amplitudes of OPs among group A - D were significant （P〈0.05）. Compared group D with group B, C, the amplitude of b-wave of Rod, Max-R and the total amplitudes of OPs were increased （P〈0.05）; latency of b-wave of Max-R was decreased （P〈0.05）. On 6 weeks after the intervention, the amplitude of b-wave of Rod and the amplitude of a-wave, b-wave of Max-R and the total amplitudes of OPs in group D were increased compared with group B and C （P〈0.05）, the latency of b-wave of Rod and Max-R in group D were decreased compared with group C （P〈0.05）. On 10 weeks after molding, each retinal layers were disordered in diabetes mellitus group. On 2 weeks after the intervention, the number of cells in the retinal layers in group B and C were reduced compared with g

关 键 词：神经干细胞/移植 糖尿病视网膜病变/治疗 动物实验 

分 类 号：R774.1[医药卫生—眼科] R587.26[医药卫生—临床医学]