黄芪注射液对模拟高原缺氧环境大鼠视网膜缺氧诱导因子-1α、p53表达的影响  

Astragalus rejection effect on the expression of hypoxia-inducible factor-1α and p53 in the retina of rats under hypoxia environment

作　　者：贾茜钰 刘勤[2] 张书[2] 臼惠玲 董文[1] 

机构地区：[1]甘肃中医药大学,兰州730000 [2]甘肃省人民医院眼科,兰州730000

出　　处：《中华眼底病杂志》2016年第4期423-427,共5页Chinese Journal of Ocular Fundus Diseases

基　　金：基金项目：甘肃省中医药管理局科研项目（GZK-2015-61）

摘　　要：目的 观察探讨黄芪注射液对模拟高原缺氧环境大鼠视网膜组织的影响及其机制。方法 成年健康Sprague Dawley大鼠60只随机分为黄芪注射液干预组（干预组）和生理盐水对照组（对照组），每组各30只。干预组大鼠按15 ml/kg剂量腹腔注射黄芪注射液，对照组大鼠腹腔注射等量生理盐水。注射后30 min，两组各按随机数字表选取6只大鼠分别饲养于模拟海拔为5000 m、氧分压11.3 kPa的高原环境模拟实验舱2、6、8、12、24 h；每一时间点均为6只大鼠。不同在舱时间大鼠出舱后处死摘除眼球，分别行石蜡切片苏木精-伊红染色和免疫组织化学染色，观察大鼠视网膜病理形态变化以及视网膜组织中缺氧诱导因子-1α（HIF -1α）、p53的表达变化。结果 对照组大鼠在舱时间2 h即可观察到视网膜各层组织疏松水肿，HIF -1α、p53主要表达于视网膜各层细胞浆中。随在舱时间延长，视网膜神经纤维层萎缩，神经节细胞肿胀变性，层次排列紊乱；HIF -1α、p53表达增加。与对照组大鼠比较，各相应在舱时间干预组大鼠视网膜组织形态改变相同，但程度更轻；HIF -1α、p53表达下降，差异均有统计学意义（P＜0.05）。结论 黄芪注射液能减轻高原缺氧环境下大鼠视网膜组织病理损害，其作用机制可能与下调HIF -1α、p53表达有关。Objective To observe the expression and mechanism of hypoxia-inducible factor-1α （HIF-1α） and p53 protein at the altitude of 5000 meter plateau hypoxia environment in rats, as well as the effect of Astragalus injection. Methods Sixty Sprague Dawley rats were randomly divided into the Astragalus injection intervention group and normal saline control group, 30 rats in each group. Astragalus injection group rats were intraperitoneal injected of Astragalus injection （15 ml/kg） before 30 minutes into the plateau environment simulation cabin, normal saline group rats were intraperitoneal injected with the same volume of saline. 30 minutes after injection, rats in each group were reared in the plateau experiment cabin which simulated altitude of 5000 m （oxygen partial pressure 11.3 kPa） for 2, 6, 8, 12, 24 hours, each time period of 6 rats. When get out, the rats were executed immediately and eyes were harvested. Retinal sections were studied by hematoxylin eosin stain, and immunohistochemical method for HIF-1α and p53 expression. Results For control rats, after 2 hours in the cabin, there was edema in retinal layers. HIF-1α and p53 were expressed mainly in the cytoplasm of retinal layers. When the periods in cabin extended, there was atrophy of retinal nerve fiber layer, swelling and degeneration of ganglion cells. The expression of HIF-1α and p53 was increased. Compared with the control group, the intervention group rat had similar but less severe retinal changes, and the expression of HIF-1α and p53 was significantly decreased （P 〈0.05）. Conclusion Astragalus injection can reduce pathological retinal damage in rats at high altitude environment, and its mechanism may be associated with reduced HIF-1α, p53 expression.

关 键 词：黄芪/药物作用 缺氧 缺氧诱导因子1 Α亚基 基因 p53 动物实验 

分 类 号：R774.1[医药卫生—眼科]