Oct4B1在结直肠癌干细胞中的表达及意义  

Expression and significance of Oct4B1 in colorectal cancer stem cells

作　　者：程家平[1] 黎江[1] 苏弦[1] 陈奕霖[1] 曾庆良[1] 文坤明[1] 

机构地区：[1]遵义医学院附属医院胃肠外科,遵义563003

出　　处：《中国免疫学杂志》2016年第6期794-797,共4页Chinese Journal of Immunology

基　　金：国家自然科学基金(No.81260369);贵州省科技厅科学技术基金[黔科合(J)字(2012)2365]资助项目

摘　　要：目的:探讨胚胎干细胞转录因子Oct4的亚型Oct4B1在结直肠癌干细胞中的表达及其可能的作用。方法:以结直肠癌细胞株SW480细胞采用悬浮培养法培养出的3D微球体及其亲本细胞SW480为研究对象,采用细胞分化实验、细胞软琼脂克隆实验、流式细胞技术检测干细胞标记物CD133、CD44的表达以验证3D微球体是否富集肿瘤干细胞(CSCs),实时荧光定量PCR(RT-q PCR)检测两种细胞Oct4B1 mRNA表达水平。结果:3D微球体具有分化为普通肿瘤细胞的能力,相对于亲本细胞,3D微球体体外克隆形成能力明显增强(P<0.01),干细胞标记物CD133、CD44明显高表达(P<0.01),Oct4B1 mRNA表达水平明显增高(P<0.01)。结论:干细胞调控因子Oct4的亚型Oct4B1在富集结直肠癌CSCs的3D微球体中表达明显增高,其可能参与结直肠癌CSCs的调控。Objective：To investigate the expression and its possible role of Oct4B1 subtype of Embryonic stem cell transcription factor Oct4 in colorectal cancer stem cells. Methods： 3D microspheres were cultured by suspension culture to human colorectal cancer cell line SW480 cells. The 3D microspheres and SW480 cells were used as the research objects. Whether 3D microspheres were enriched cancer stem cells,we used the methods of cell differentiation experiments,soft agar cloning experiments,and the expression levels of cancer stem cells markers CD133,CD44 detected by flow cytometry. The expression levels of Oct4B1 mRNA were detected by RT-qPCR. Results：3D microspheres could differentiate into normal cancer cells. Compared with the parental SW480 cells,in vitro colony formation was significantly enhanced（P〈0. 01）,the percentage of positive cells of CD133 and CD44 were significantly increased （ P 〈 0. 01 ）, the expression levels of Oct4B1 mRNA were obviously higher （ P 〈 0. 01 ） in 3D microspheres. Conclusion： Oct4B1 subtype of Embryonic stem cell transcription factor Oct4 in 3D microspheres enriched human colorectal cancer stem cells,which may be involved in the regulation of colorectal cancer stem cells.

关 键 词：结直肠癌 肿瘤干细胞 OCT4 Oct4B1 

分 类 号：R735.3[医药卫生—肿瘤]