上调miRNA-145对肺癌细胞系A549增殖和凋亡的影响  

Effects of Up-regulated microRNA-145 on proliferation and apoptosis of human lung cancer cell line A549

作　　者：徐驰[1,2] 汪栋[1,3] 苏长青[4] 杨胜生[2] 张剑锋[1,3] 叶玉坤[1,3] 

机构地区：[1]第二军医大学八一医院临床学院,南京210002 [2]第二军医大学福州总医院临床医学院,福州350025 [3]中国人民解放军第八一医院,南京210002 [4]第二军医大学东方肝胆外科医院分子肿瘤研究室,上海200438

出　　处：《中国肿瘤临床与康复》2016年第7期769-772,共4页Chinese Journal of Clinical Oncology and Rehabilitation

基　　金：军队临床高新技术重大项目(2010gxjs 027);福建省科技计划项目(2012D021);福建省自然科学基金(2015J01486)

摘　　要：目的探讨miRNA-145对肺癌细胞系A549增殖和凋亡能力的影响。方法采用实对荧光定量聚分酶链反应检测A549细胞中miRNA-145的表达,并将miRNA-145表达量上调(实验组)与空白对照组和阴性对照组比较,采用CCK8法检测并对比A549细胞增殖的改变。采用流式细胞仪检测并对比A549细胞周期以及凋亡的改变。结果 RT-PCR结果表明,侵染了miRNA-145过表达载体的A549细胞中miRNA-145水平明显高于阴性对照组及空白对照组,差异有统计学意义(P<0.01);上调A549细胞中miRNA-145的表达量,可使A549细胞出现S期阻滞,凋亡增加、增殖减缓(P<0.05)。结论过表达miRNA-145能够抑制A549细胞的增殖并诱导A549细胞的凋亡,有可能为肺癌分子靶向治疗调控效应靶点作进一步探索研究。Objective To investigate the effects of miRNA-145 on proliferation and apoptosis of human lung cancer cell line A549. Methods A549 cells were transfeeted by lentiviral expression vector of miRNA-145 gene. Cell cycle and apoptosis were detected by flow cytometry and proliferation were detected by CCK-8 assay after transfection. Results The result of real-time RT-PCR showed that miRNA-145 expression in A549 cell infected by hsa-miR-145-Sp was higher than others （P 〈0. 01 ）. S phase cycle was arrested and apoptosis was increased and proliferation was decreased after up-regulation of miRNA-145 in A549 cell（ P 〈 0.05 ）. Conclusion Up-regulated miRNA-145 could suppress proliferation and increase apoptosis, it may be helpful for further investigation of the regulation of the target effect for the molecular target therapy of lung cancer.

关 键 词：癌 非小细胞肺 miRNA-145 细胞增殖 凋亡 细胞周期 

分 类 号：R734.2[医药卫生—肿瘤]