ShRNA靶向沉默STX8基因对U251细胞增殖和迁移侵袭的影响  

Effect of Sh RNA targeted silencing of STX8 gene on proliferation and migration of U251 cells

作　　者：杨海峰[1] 杨亮[2] 毕艳华[3] 黄淑红[4] 

机构地区：[1]北京市仁和医院神经外科,北京102600 [2]河北医科大学第二医院神经外科,河北石家庄050000 [3]华北石油管理局总医院科研教学培训科,河北任丘062550 [4]山东大学医学院神经生物学系,山东济南250012

出　　处：《中国医药导报》2016年第19期4-6,30,共4页China Medical Herald

基　　金：国家自然科学基金资助项目(31271519)

摘　　要：目的探讨敲低STX8基因对脑胶质瘤细胞瘤U251细胞增殖、迁移和侵袭的影响。方法构建STX8-sh RNA-p Super表达载体转染U251细胞,分别用CCK8法、划痕实验、Transwell实验检测细胞增殖、迁移侵袭能力。结果 CCK8法、划痕实验、Transwell实验结果显示,STX8干扰后细胞增殖、迁移侵袭能力显著下降。结论 STX8基因的敲低能有效抑制U251细胞增殖、迁移侵袭能力,STX8基因有可能成为胶质母细胞瘤基因治疗的新靶点。Objective To study the effect of knockdown STX8 gene on proliferation and migration of U251 cells. Methods STX8-shRNA-pSuper expression vector, transfected U251 cells were constructed, cell proliferation, migration and invasion ability were determined by CCK8 test, scratch test and transwell assay respectively. Results The cell proliferation, migration and invasion ability of STX8 interference group was significantly decreased. Conclusion Knockdown of STX8 inhibits the proliferation, migration and invasion ability of U251 cells, STX8 gene may be a new target for gene therapy of glioblastoma.

关 键 词：STX8基因 胶质细胞瘤 RNA干扰 U251细胞 

分 类 号：R739.41[医药卫生—肿瘤]