亚麻子水提液对DPP-4及α-葡萄糖苷酶活性抑制实验研究  

Experimental Study of Inhibit Activity of DPP-4 and α-glucosidase with Flaxseed Water Extraction Liquid

作　　者：张碧溦 李继安 田春雨 周雪梅 王晨斌 徐雪梅 焦子珊 喇孝瑾 

机构地区：[1]华北理工大学中医学院,河北唐山063000

出　　处：《云南中医学院学报》2016年第3期14-16,共3页Journal of Yunnan University of Traditional Chinese Medicine

基　　金：河北省国际合作项目(14397705D);河北省中医药管理局科研计划项目(2013066)

摘　　要：目的通过观察亚麻子水提液对二肽基肽酶-4(DPP-4)、α-葡萄糖苷酶抑制作用,为该药防治糖尿病提供实验依据。方法 1以DPP-4酶、缓冲液、底物建立DPP-4抑制剂的体外筛选体系,对亚麻子水提液进行抑制实验,采用发色底物法测定吸光度(OD),计算DPP-4抑制率及IC50值;2以蔗糖为底物建立α-葡萄糖苷酶活性抑制模型,采用葡萄糖氧化酶法测定亚麻子水提液对α-葡萄糖苷酶的抑制作用,计算其抑制率和IC50值。结果 1亚麻子具有轻度DPP-4抑制作用,其IC50值738.20mg/L;2亚麻子具有α-葡萄糖苷酶抑制作用,其IC50值为365.9mg/m L。结论亚麻子水提液可一定程度地抑制DPP-4及α-葡萄糖苷酶活性。Objective Provide experimental basis through observing inhibit activity of DPP-4 and a-glucosidase with flaxseed water extraction liquid. Methods 1. Established in vitro screening system of DPP-4 inhibitor by using DPP-4 enzyme,buffer solution and substrate. Then carried on suppression experiment of flaxseed water extraction liquid and determined absorbanee through chromogenic peptide substrate method. Finally,calculated the DPP-4 suppression rate and the IC50 value. 2. Took sucrose as substrate to establishot-glucosidase enzyme activity inhibition model. Then determined inhibitory effect of flaxseed water extraction liquid on a-glucosidase enzyme. Finally,calculated the inhibition rate and IC50 value. Results 1. Flaxseed can mildly inhibit DPP- 4,its IC50 value was 738.20mg/L. 2. Flaxseed can inhibit a-glucosidase enzyme,its IC50 value was 365.9mg/mL. Conclusion Flaxseed water extraction liquid caninhibit the activity of DPP-4 and alpha glycosidase enzyme to a certain degree.

关 键 词：亚麻子 DPP-4 Α-葡萄糖苷酶 2型糖尿病 

分 类 号：R285.5[医药卫生—中药学]