环氧化酶2基因rs689466位点与脑梗死患者阿司匹林抵抗的关系  

Association between rs689466 in COX-2 gene and aspirin resistance in cerebral infarction patients

作　　者：郭文洁[1] 毛诗贤[2] 张德炳 陈泽雯[1] 冯占辉[1] 楚兰[1] 

机构地区：[1]贵州医科大学附属医院神经内科,贵阳市550014 [2]贵州医科大学附属白云医院神经内科,贵阳市550014

出　　处：《实用医学杂志》2016年第13期2079-2083,共5页The Journal of Practical Medicine

基　　金：贵州省卫生厅科学技术基金项目(编号:黔科合LG字【2012】067号)

摘　　要：目的:探讨环氧化酶2(COX-2)基因上的SNP位点和阿司匹林抵抗的关系。方法:150例脑梗死患者纳入本研究。血小板聚集反应通过光透射法测定。血清学指标通过检测空腹静脉血得到。COX-2基因上的4个SNP通过测序方法进行基因分型。结果:150例患者中,60例被定义为阿司匹林抵抗者。临床指标中,阿司匹林抵抗组的同型半胱氨酸浓度和脑梗死复发频率显著高于阿司匹林反应组。在针对SNP的单变量分析中,rs20417、rs689465和rs689466与阿司匹林抵抗显著相关。多变量分析中,在矫正了其他SNP和临床因素后,rs20417和rs689466依然与阿司匹林抵抗显著相关。结论:在矫正了rs20417的作用后,rs689466仍然与阿司匹林抵抗显著相关。通过联合检测rs689466、rs20417和其他临床因素,能够更好地区分出阿司匹林抵抗者。Objective To investigate the association of single nucleotide polymorphisms （SNPs） in COX- 2 with aspirin resistance in Chinese cerebral infarction patients. Methods A total of 150 Chinese cerebral infarction patients were recruited. Platelet aggregation response was measured by light transmission aggregometry method and four SNPs located in COX2 gene were genotypcd by sequencing method. Results Sixty patients of the total were classified as aspirin non-responders. For clinical variables, concentrations of high homocysteine and the frequency of recurrence cerebral infarction were significantly higher in aspirin non-responders when compared with aspirin responders. Univariate analysis of SNPs showed that rs20417, rs689465 and rs689466 were significantly associated with aspirin resistance. Multivariate analysis indicated that after adjusting other SNPs and clinical risk factors, rs20417 and rs689466 were still significantly associated with aspirin resistance. Conclusions Rs689466 is significantly associated with aspirin resistance in Chinese cerebral infarction patients even after the adjustment of rs20417. By combining rs689466, rs20417 and other clinical risk factors, we may better classify the aspirin non-responders from aspirin responders.

关 键 词：脑梗死 环氧化酶2 单核苷酸多态性位点 阿司匹林抵抗 

分 类 号：R743.3[医药卫生—神经病学与精神病学]