内质网应激分子GRP78在不同时程吗啡依赖大鼠睾丸中的表达  

Expression of endoplasmic reticulum stress-associated molecule GRP78 in testicular tissue of rats in different phases of morphine-dependence

作　　者：吴明松 罗素元[2] 郑翔[3] 涂平[2] 白威峰[2] 刘兴宇[2] 

机构地区：[1]贵州省高等学校口腔疾病研究特色重点实验室,医学与生物学研究中心,遵义市563000 [2]遵义医学院细胞生物学教研室,遵义市563000 [3]遵义医学院医学遗传学教研室,遵义市563000

出　　处：《实用医学杂志》2016年第13期2108-2112,共5页The Journal of Practical Medicine

基　　金：贵州省科技厅社会公关项目(编号:黔科合SY字[2013]3065);遵义医学院青年基金项目[编号:(2007)014,(F-240)]

摘　　要：目的:通过观察内质网应激标志分子葡萄糖调节蛋白78(GRP78)在不同时程吗啡依赖大鼠睾丸中的表达变化,探索内质网应激在阿片类药物成瘾中的作用。方法:SD大鼠分为生理盐水(NS)戒断组、NS消退组、NS点燃组、吗啡(Mor)戒断组、Mor消退组、Mor点燃组,前3组注射NS,后3组皮下注射Mor,建立条件性位置偏爱(CPP)大鼠模型。分别于戒断48 h、消退和用吗啡点燃时,取大鼠的睾丸组织,用免疫印迹法检测GRP78水平。结果:吗啡给药10 d并戒断48 h后,大鼠在伴药箱中的时间为(528.0±81.0)s,显著高于NS对照组(P<0.001),经过17 d自然戒断后在伴药箱中的时间为(396.8±116.9)s,显著低于其戒断时的时间(P<0.001),用吗啡点燃后在伴药箱中的时间为(530.7±91.9)s,显著高于消退时的时间(P<0.001),表明吗啡消退、点燃模型建立成功。GRP78在戒断48 h时表达下调(P<0.05)、消退时表达增加且略高于NS消退组;在点燃时,GRP78水平急剧上调(P<0.01)。结论:睾丸内质网应激参与了吗啡成瘾,内质网应激在阿片类药物成瘾者男性生殖功能紊乱中可能扮演重要角色。Objective To investigate expression level of endoplasmic reticulum stressmarker GRP78 in the testicular tissue in rats with different phases of morphine-dependence. To explore the role of ERS in morphine-de- pendence. Methods SD rats were divided into 6 groups： morphine （mor） -withdrawal group, mot-extinct group, mot-kindling group and their control groups, normal saline （NS）-withdrawal group, NS-extinet group, NS-kindling group. The experimental rats were injected with morphine subcutaneously on increasing dosage to establish the con- ditioned place preference （CPP） model. The rats in control groups were injected NS. Then the rats were suffered from withdrawal for 48 h, extinction and kindling by morphine, separately. The GRP78 expression level in testicular tissues of rats in the time point mentioned above were measured using Western Blot. Results The time of rats in the paired-box was （528.0 _＋ 81.0） s, which was significantly higher than that in the NS control group （P 〈 0.001）. It was （396.8 ± 116.9） s after extinctive phase, which was significantly higher than that the withdrawal phase of rats （P 〈 0.001）. Also it was （396.8 ± 116.9） s after kindling with morphine which was significantly higher than that the extinctive phase of rats （P 〈 0.001）. These changes of the time indicated that the animal models of extinction and kindling were established in the study. The GRP78 levels were down-regulated in 48 h after withdrawal （P 〈 0.05）, and increased a bit afterextinctive phase, but up-regulated highly after kindling with morphine （P 〈 0.01）. Conclusion ERS may be related in the morphine dependence and it might play an important role of testicular dys- function in male drngabuser.

关 键 词：药物依赖 吗啡 内质网应激 GRP78 睾丸 

分 类 号：R749.6[医药卫生—神经病学与精神病学]