外周血Tcm和Treg在胰腺癌中的表达及其与临床病理因素的关系  

Expression and Clinical Significance of Peripheral Blood Central Memory T Cells and Treg in Pancreatic Cancer

作　　者：郑丽娜 李则学[2] 张兰[2] 梁凯[2] 梁文涛[2] 刘海亮[2] 

机构地区：[1]解放军陆军总医院门诊部,北京100700 [2]解放军总医院全军普通外科研究所,北京100853

出　　处：《临床误诊误治》2016年第7期84-87,共4页Clinical Misdiagnosis & Mistherapy

基　　金：国家自然科学基金(81172891)

摘　　要：目的探讨胰腺癌患者外周血中央型记忆T细胞(central memory T cells,Tcm)和调节性T细胞(T regulatory cells,Treg)的表达情况,及其与临床病理因素的关系。方法选取2014年6月—2016年1月解放军总医院普通外科收治的128例未治胰腺癌患者作为胰腺癌组,另选取同期48例体检健康者作为健康对照组。两组均采集外周血5 ml,分离单个核细胞,以荧光标记的抗CD3、CD4、CD8、CD45RO、CD62L、CD25、FOXP3抗体染色,运用流式细胞术检测Tcm及Treg表达率。并进一步分析胰腺癌组Tcm和Treg表达及其与临床病理因素的关系。结果胰腺癌组Tcm及Treg表达率均显著高于健康对照组[(19.38±5.42)%vs(12.22±3.21)%,t=8.60,P=0.00;(2.34±0.89)%vs(0.17±0.03)%,t=16.87,P=0.00]。胰腺癌组Tcm及Treg表达率均与临床分期及淋巴结转移相关(P均<0.05),Tcm表达率还与患者年龄相关(χ2=28.00,P=0.00)。结论未治胰腺癌患者外周血Tcm和Treg高表达,提示存在一定程度的抗肿瘤预存免疫记忆,但呈现为免疫抑制状态;其表达与肿瘤临床分期及淋巴结转移相关。Objective To analyze clinical characteristics and significance of central memory T cells and Treg cells of patients with pancreatic cancer. Methods 128 patients with pretreatment pancreatic cancer in our hospital and 48 healthy volunteers were enrolled into this study between June 2014 and January 2016. The percentage of central memory T cells （Tcm, CD8 ＋ CDd5RO ＋ CD62L ＋ ） and Treg （CD3 ＋ CD4 ＋ CD25 ＋ FOXP3 ＋ ） in the peripheral blood were assessed through flow cytometry with fluorescence-labelled CD3, CD4, CD8, CD45RO, CD62L, CD25, FOXP3 antibody. The relationship of central memory T cells and Treg cells with clinical pathologic characteristics of pancreatic cancer was also evaluated. Results Compared to healthy volunteer, the mean percentage of Tcm and Treg in pancreatic cancer patients increased significantly [Tcm, （19.38±5.42）% vs （12.22±3.21）%,t =8.60,P =0.00; Treg, （2.34±0.89）% vs （0.17 ±0. 03） % ,t = 16.87,P = 0.00 ] , which were related to the lymph nodes involvement and clinical stage （P 〈 0.05 ） , otherwise, the percentage of Tcm was associated with the age of patients （ X2 = 28.00, P = 0.00）. Conclusion Pancreatic cancer patients show high Tcm and Treg expressions in peripheral blood associated with clinical stage and lymph nodes involvement, which indicates the immunosuppressant status and limited pre-existing anti-tumor immunity.

关 键 词：胰腺肿瘤 T淋巴细胞亚群 T淋巴细胞 调节性 基因表达 

分 类 号：R735.9[医药卫生—肿瘤]