DLL4和TMPRSS4在非小细胞肺癌中的表达及临床意义  

Expressing and Clinical Significance of DLL4 and TMPRSS4 in Non-small Cell Lung Cancer

作　　者：王维[1] 池菲[1] 栾艳超[1] 白峰[1] 李强[1] 

机构地区：[1]河北省胸科医院胸外科,石家庄050041

出　　处：《临床误诊误治》2016年第7期105-108,共4页Clinical Misdiagnosis & Mistherapy

基　　金：河北省卫生厅科研基金项目(20130446)

摘　　要：目的探讨Delta样配体4(delta-like ligand4,DLL4)和Ⅱ型跨膜丝氨酸蛋白酶4(typeⅡteansmembrane serine 4,TMPRSS4)在非小细胞肺癌(non-small cell lung cancer,NSCLC)中的表达及其临床意义。方法选取手术前未行放化疗的NSCLC患者72例癌组织标本作为观察组,另选取距离原发肿瘤5 cm以上、经病理证实无肿瘤浸润的肺组织标本作为正常对照组。通过免疫组织化学染色法,检测两组DLL4和TMPRSS4表达情况,分析观察组DLL4和TMPRSS4表达与患者临床病理特征的关系以及二者表达的相关性。结果观察组DLL4阳性表达率为65.3%,TMPRSS4阳性表达率为79.2%,均高于正常对照组的25.0%和26.4%,差异均有统计学意义(P均<0.01)。观察组DLL4和TMPRSS4的表达与患者肿瘤临床分期、分化程度、淋巴结转移情况相关(P<0.05或P<0.01)。Spearman相关性分析显示,观察组DLL4与TMPRSS4表达呈正相关(r=0.344,P=0.003)。结论 DLL4和TMPRSS4在NSCLC的发生、发展和转移中可能起协同作用,DLL4联合TMPRSS4检测对判断NSCLC预后有一定价值。Objective To investigate the correlation of DLL4 and TMPRSS4 in human non-small cell lung cancer and their clinical significance. Methods 72 specimens of NSCLC without radiation and chemotherapy before operation were selected as observation group and specimens without tumor invasion and with the lung tissues within 5 centimeters from primary tumor were selected as normal control group. We detected the expression of DLIA and TMPRSS4 in the two groups, and analyzed the relationship between the expression of DLL4 and TMPRSS4 and the clinicopathological characteristics, using the method of immnnohistochemical staining. Results The positive expression rate of DLIA in observation group was 65.3% , TMPRSS4 positive rate was 79.2% , higher than that in normal control group of 25.0% and 26.4% respectively, and the difference was statistically significant （P 〈 0. 01 ）. The expression of DLIAt and TMPRSS4 in observation group related to clinical stages, differentiation degree and lymph node metastasis （P 〈 0. 05 or P 〈 0. 01 ）. Spearman correlation analysis showed that the expression of DLL4 was positively correlated with TMPRSS4 expression in the observation group （ r = 0. 344, P = 0. 003）. Conclusion The DLL4 and TMPRSS4 may play a collaborative role in occurrence, development and metastasis in NSCLC. Detecting the expression of DLL4 combined with TMPRSS4 for clinical judgment against non-small cell lung cancer prognosis is of certain value.

关 键 词：癌 非小细胞肺 DLL4 TMPRSS4 免疫组织化学 基因表达 

分 类 号：R734.2[医药卫生—肿瘤]