基质细胞衍生因子-1调控髓核细胞基质金属蛋白酶-13表达的机制  

The research on the mechanism of stromal cell - derived factor - 1 regulating the expression of matrix metalioproteinase -13 in nucleus pulposus cells

作　　者：李帅[1] 郜勇[1] 王琨[1] 刘伟[1] 宋雨[1] 陈松峰[1] 杨操[1] 

机构地区：[1]华中科技大学同济医学院附属协和医院骨科,武汉430022

出　　处：《中华实验外科杂志》2016年第7期1701-1703,共3页Chinese Journal of Experimental Surgery

摘　　要：目的探讨基质细胞衍生因子-1（SDF-1）对髓核细胞基质金属蛋白酶-13（MMP-13）表达的调控机制。方法体外培养大鼠髓核细胞，应用SDF-1进行干预，以CXC趋化因子受体4、7（CXCR4、CXCR7）中和抗体阻抑SDF-1／CXCR4、7轴，检测MMP-13和活性的改变，荧光素酶报告基因检测法验证SDF-1与MMP-13作用关系。结果SDF-1促进髓核细胞MMP-13表达，CXCR4中和抗体抑制MMP-13的表达和活性改变（P〈0．05）。SDF-1增加MMP-13启动子的活性2．7倍，CXCR4抑制剂可降低MMP-13启动子活性3．8倍（P〈0．05）。结论SDF-1结合CXCR4后，激活MMP-13启动子并上调其表达水平。Objective To investigate the mechanism of stromal cell derived factor - 1 （ SDF - 1 ） regulating the expression of matrix metalloproteinase - 13 （ MMP - 13 ）. Methods Rat nucleus pulposus （NP） cells were isolated and co - cuhured with SDF - 1. CXC chemokine receptor - 4 （ CXCR4 ） and CXCR7 - neutralizing antibody were incubated respectively to inhibit the SDF - 1/CXCR4 and SDF - 1/ CXCR7 axis. The expression of MMP - 13 and activity was detected. Lueiferase activity was measured with the Lueiferase reporter assay system to investigate the correlation between SDF - 1 and MMP - 13. Results SDF- 1 induced the expression of MMP - 13, while the expression of MMP - 13 could be blocked by CXCR4 neutralizing antibody （ P 〈 0. 05 ）. SDF - 1 increased the lueiferase activities of MMP - 13 promot- er by 6. 2 - fold. Addition of CXCR4 inhibitor reduced the lueiferase activities by 6. 2 - fold （ P 〈 0.05 ）. Conclusion SDF - 1 acted through CXCR4 to increase MMP - 13 promoter activity and upregulate MMP - 13 expression.

关 键 词：基质细胞衍生因子-1 基质金属蛋白酶-13 髓核细胞 启动子 CXC趋化因子受体4 

分 类 号：R363.2[医药卫生—病理学]