Wnt／β-连环蛋白信号通路在皮质骨和松质骨表达的差异  

The difference of Wnt/β- catenin signaling pathway expression in cortical and trabecular bone

作　　者：李俊峰[1,2] 陈思旭[1] 鲍全伟 刘华渝[1] 李昂[1] 秦昊[1] 赵玉峰[1] 沈岳[1] 钟孝政[1] 宗兆文[1] 

机构地区：[1]第三军医大学大坪医院野战外科研究所创伤科创伤烧伤与复合伤国家重点实验室,重庆400042 [2]解放军第180医院(泉州海峡医院)骨科,泉州362008

出　　处：《中华实验外科杂志》2016年第7期1707-1710,共4页Chinese Journal of Experimental Surgery

基　　金：国家自然科学基金（81271935）;创伤烧伤与复合伤国家重点实验室自主课题（SKLZZ201124）;“十二五”科研项目重大项目分课题（AWSllJ008）

摘　　要：目的探讨皮质骨和松质骨Wnt／β-连环蛋白（B—catenin）信号通路及相关分子表达的差异。方法通过小鼠在体和离体实验，观察皮质骨和松质骨Wnt／β-catenin通路及相关因子B—catenin、核心结合蛋白因子2（Runx2）、成骨相关转录因子抗体（Osterix）等表达水平的差异。结果免疫组织化学结果显示小鼠皮质骨B—catenin、Runx2、Osterix阳性表达量均明显低于松质骨l（20．30±1．21）％比（25．80±1．10）％、（10．20±3．05）％比（19．51±2．03）％、（13．90±2．06）％比（23．74±3．33）％，P〈0．05]。细胞免疫荧光和实时定量聚合酶链反应（Real—time PCR）的结果与免疫组织化学结果一致。结论皮质骨的Wnt／β-catenin信号通路及相关分子表达明显低于松质骨。Objective To investigate the difference of Wnt/β- catenin pathway and associating factorsexpression in cortical and trabecularbone. Methods β- catenin and associating factors such as related transcription factor - 2 （ Runx2）, Osterixin in cortical and trabecular bone were examined in vitro and in vivo. Results Immunohistochemical staining on micerevealed that the expression levels of 13 - catenin, Runx2 and Osterix in cortical bone were significantly lower than those in trabecular bone [ （ 20. 30 ± 1.21）% vs. （25.80±1.10）%, （10.20±3.05）% vs. （19.51 ±2.03）%, （13.90±2.06）% vs. （23.74 ±3.33）% ; P 〈0. 051. The results of cellular immune fluorescence and real - time quantitative polymerase chain reaction （Real -time PCR） were identical with the Immunohistochemieal staining. Conclusion The activity of Wnt/β - catcnin signaling pathway and associating factors expressionin cortical bone were significantly lower than those in trabecular bone.

关 键 词：皮质骨 松质骨 Wnt/β-连环蛋白信号通路 

分 类 号：R730[医药卫生—肿瘤]