OSI-027抑制肝内胆管细胞癌的增殖和增强顺铂对肝内胆管细胞癌杀伤作用及其机制  

OSI -027 inhibited proliferation of intrahepatic cholangiocarcinoma and enhanced the eytotoxicity of cisplatin in vitro

作　　者：薛飞[1] 刘艳慧[2] 张宏伟[1] 闻愚 楚皓源 唐强[1] 王玉柱[1] 郭志松[3] 

机构地区：[1]河南省人民医院郑州大学人民医院肝胆胰腺外科,郑州450003 [2]河南省人民医院郑州大学人民医院血液内科,郑州450003 [3]河南省人民医院郑州大学人民医院重症医学部,郑州450003

出　　处：《中华实验外科杂志》2016年第7期1771-1773,共3页Chinese Journal of Experimental Surgery

基　　金：国家自然科学基金（81273260）

摘　　要：目的观察OSl-027对肝内胆管细胞癌细胞株RBE和HCCC-9810增殖的影响和OSI-027与顺铂联用在肝内胆管细胞癌治疗中的作用及其机制。方法分别用OSI-027、顺铂、OSI-027和顺铂联用处理肝内胆管细胞癌细胞株RBE、HCCC-9810。细胞计数试剂盒（CCK-8）实验检测细胞活力，EdU实验检测细胞增殖，流式细胞术检测细胞凋亡。对照组为不做任何处理的RBE、HCCC-9810细胞。结果OSI-027可以显著抑制RBE和HCCC-9810的细胞活力和增殖，表现为显著的时间和浓度依赖性。单独使用OSI-027不能诱导RBE和HCCC-9810凋亡，而单独使用顺铂能够诱导RBE和HCCC-9810凋亡[RBE：（6．56±1．27）％，HCCC-9810：（9．25±1．49）％]。OSI-027与顺铂联用显著增强顺铂诱导的RBE和HCCC-9810细胞凋亡[RBE：（16．78±2．23）％，HCCC-9810：（21．47±2．17）％]。结论OSI-027可以抑制肝内胆管细胞癌的增殖，其与顺铂联用后可增强顺铂对肝内胆管细胞癌的杀伤作用。Objective To investigate the effect and mechanism of OSI -027 combined with cispl- atin on proliferation and apoptosis of human intrahepatic eholangiocarcinoma （ ICC ） cell lines RBE and HCCC- 9810. Methods RBE and HCCC -9810 cells were treated with OSI -027, cisplatin, or OSI -027 plus cisplatin, respectively. Cell counting kit -8 （CCK -8 ） kit was used to test cell viability. The proliferation and apotosis of ICC cell lines were examined by Ethynyl deoxyuridine （EdU） assay and flow - cytomery, separately. The cells not given treatment served as control group. Results OSI - 027 significantly inhibited the proliferation of RBE and HCCC - 9810 cells in a concerntration - and time - dependent manner. Cisplatin, but not OSI -027, induced apoptosis of RBE and HCCC -9810 cells [ RBE： （6. 56 ±1.27） % , HCCC - 9810 ： （9. 25 ± 1.49） % ]. When OSI - 027 combined with cisplatin was given, apopotosis of ICC cells occurred apparently [ RBE ： （ 16. 78 ± 2. 23 ） %, HCCC - 9810 ： （21.47 ± 2. 17）% ]. The combination of cisplatin and OSI -027 displayed more efficient in vitro. Conclusion OSI -027 suppressed proliferation of ICC cell lines and enhanced apotosis induced by cisplatin in vitro.

关 键 词：OSI-027 顺铂 肝内胆管细胞癌 增殖 脱噬作用 

分 类 号：R735.7[医药卫生—肿瘤]