配对相关同源框-1基因过表达对人胰腺癌细胞侵袭及上皮-间充质转化的影响  

Effects of overexpression of paired - related homeobox 1 gene on invasion and epithelial mesenchy- mai transition of human pancreatic cancer cells

作　　者：范钰[1] 蒋孟林 满昌峰[1] 臧晔 邹晨[1] 

机构地区：[1]江苏省江苏大学附属人民医院肿瘤研究所,镇江212002 [2]江苏省丹阳市人民医院肿瘤科,丹阳213000

出　　处：《中华实验外科杂志》2016年第7期1789-1791,共3页Chinese Journal of Experimental Surgery

基　　金：江苏省省级重点研发专项基金（BE2015666）;镇江市卫生科技重点专项项目（SHW2015004、SHW2015006）;江苏大学临床科技基金（JLY20140002）

摘　　要：目的观察配对相关同源框-1（Prrx-1）基因过表达对胰腺癌细胞侵袭及上皮-间充质转化（EMT）的影响。方法培养人正常胰腺导管上皮细胞HPDE6-C7和3株胰腺癌细胞AsPC-1、BxPC-3和CFPAC-1，采用Westernblot方法检测Prrx-1蛋白表达。采用Prrx-1基因过表达慢病毒载体转染胰腺癌BxPC-3细胞，构建高表达Prrx-1基因过表达BxPC-3细胞（简称Prrx-1过表达细胞）后，采用Westernblot方法检测癌细胞Prrx-1蛋白水平；采用显微镜观察癌细胞形态；采用Westernblot方法检测各组癌细胞中上皮标志物E-钙黏蛋白（E—cadherin）和间叶标志物波形蛋白（Vimentin）蛋白；采用Transwell方法观察癌细胞侵袭能力。结果Westernblot检测结果显示：Prrx-1蛋白在HPDE—C7细胞不表达，而在AsPC-1、BxPC-3和CFPAC-1均高表达。选取BxPC-1进一步研究。采用Westernblot方法检测结果显示，与空载对照组比较，Prrx-1过表达组细胞Prrx-1基因蛋白水平明显升高；形态学观察结果显示，Prrx-1基因过表达组细胞出现EMT改变；Westernblot检测结果显示，与空载对照组比较，Prrx-1过表达组细胞E—Cadherin表达下调，Vimentin表达上调；Transwell方法检测发现，空载对照组和Prrx-1过表达组穿膜细胞数分别为（89±5）个和（139±8）个（P〈0．05）。结论Prrx．1过表达可促进人胰腺癌细胞迁移和侵袭能力，其机制可能与促进EMT有关。Objective To observe the effects of paired - relatedhomeobox 1 （ Prrx - 1 ） gene overexpression on invasion and epithelial mesenchymal transition of pancreatic cancer cells. Methods Human normal pancreatic ductal epithelial ceils HPDE6 - C7 and pancreatic cancer AsPC - 1, BxPC - 3 and CFPAC - 1 cells were cultured, and Western blotting method was used to detect the expression of Prrx - 1 protein. Prrx - 1 overexpression of pancreatic cancer BxPC - 3 ceils was constructed by overexpression of slow virus vector（ Referred to as Prrx - 1 overexpression cells）. The protein of Prrx - 1, and E - cadherin, and Vimentin in cancer cells were detected of by Western blotting. The morphology of cancer cells was observed by microscope. The invasion ability of cancer cells was observed by transwell method. Results Prrx - 1 protein was rare in HPDE - C7 cells, while Prrx - 1 protein was highly expressed in AsPC - 1, BxPC - 3 and CFPAC - 1. BxPC - 1 was used as a further study. The level of Prrx - 1 protein in Prrx - 1 overexpressed group was significantly higher than that in the control group. Morphological observation showed that Prrx- 1 overexpression group cells appear epithelial- mesenchymal transition （EMT） change. Compared with the blank control group, the E - cadherin protein in Prrx - 1 overexpression group was down - regulated ; while the vimentin protein was up - regulated. The results of transwell showed that the number of cells across the membrane of control group and Prrx - 1 overexpressed group was 89 ± 5, and 139 ±8, respectively （ P 〈 0. 05 ）. Conclusion Prrx - 1 overexpression could promote invasion of human pancreatic cancer cells which might be related to the promotion of epithelial mesenchymal transition.

关 键 词：胰腺癌 配对相关同源框1 侵袭 上皮-间充质转化 

分 类 号：R737.14[医药卫生—肿瘤]