核糖核酸腺苷脱氨酶1在肝癌中的表达及临床意义  

Expression and influence of adenosine deaminase acting on RNA 1 in primary liver cancer

作　　者：张慧峰[1] 秦秉玉[1] 余海波[2] 

机构地区：[1]河南省人民医院(郑州大学人民医院)重症医学科,郑州450003 [2]河南省人民医院(郑州大学人民医院)肝胆胰外科,郑州450003

出　　处：《中华实验外科杂志》2016年第7期1830-1832,共3页Chinese Journal of Experimental Surgery

基　　金：河南省科技厅基础与前沿技术项目（112300410037）;河南省重点科技攻关计划项目（132102310385）;郑州市重点实验室项目（2015-9-23）;河南省医学科技攻关计划重点项目（201502018）

摘　　要：目的探讨核糖核酸腺苷脱氨酶1（ADAR1）在原发性肝癌中的表达及其与肝癌临床病理特征的关系。方法免疫组织化学及Western blot法检测ADARl在癌旁组织及肝癌肿瘤组织中表达；收集患者年龄、性别、肿瘤直径、门脉癌栓、肝背膜受侵、分化程度、血清甲胎蛋白（AFP）及细胞核增殖抗原（Ki-67）的表达，了解ADAR1与肝癌临床病理特征的关系。结果免疫组织化学检测结果显示，ADAR1在癌旁组织中ADAR1表达率为29．32％，而在肝癌肿瘤组织中表达率为64．50％，肿瘤组织ADAR1阳性率远高于癌旁组织，差异有统计学意义（P〈0．05）；Western blot结果表明，癌组织ADAR1表达量明显高于癌旁组织，差异有统计学意义（P〈0．05）；肝癌组织中的ADAR1表达水平与患者年龄、性别、肿瘤直径、门脉癌栓及肝背膜受侵差异无统计学意义（P〉0．05），而与分化程度、血清AFP及Ki-67的表达密切相关，ADAR1表达水平明显升高（P〈0．05）。结论ADARl在肝癌中表达增加，且与肝癌分化程度和血管生成基因相关，提示ADARl可能通过诱导肝癌分化及血管生成而促进肝癌发生侵袭转移。Objective To investigate adenosine deaminase acting on RNA 1 （ ADAR1 ） in primary liver cancer （PLC） and its relationship with clinical pathology. Methods ADAR1 expression was detected by immunohistochemistry and Western blotting assay in cancer tissues and tumor adjacent tissue; age, gender, tumor size, portal vein tumor thrombus, liver back film invasion, differentiation, serum alpha fetal protein （AFP） and proliferation cell nuclear antigen （Ki -67 ） expression were collected to explore relationship of ADAR1 and clinical pathology. Results Immunohistochemical detection showed that ADAR1 expression rate of tumor adjacent tissue was 29. 3%, and tumor tissue was 64. 50%, there was significant difference of ADARI positive rate between tumor tissue and adjacent tissue （ P 〈 0. 05 ） ; Western blotting assay results showed that ADAR1 expression in cancer tissue was significantly higher than that in the tumor adjacent tissue, the difference was statistically significant （ P 〈 0. 05 ） ; There were no statistical significance between ADAR1 expression levels and patient age, gender, tumor size, portal vein tumor thrombus and liver back film invasion showed （ P 〉 0.05 ） , and the degree of differentiation, serum AFP and Ki - 67 expression is closely related to that ADAR1 expression level increased significantly （ P 〈 0.05 ）. Conclusion ADARI expression in PLC increased associated with tumor differentiation and angiogenesis gene, suggesting that ADAR1 may induce degree of differentiation and angiogenesis to promote liver cancer invasion.

关 键 词：原发性肝癌 核糖核酸腺苷脱氨酶1 侵袭 转移 

分 类 号：R735.7[医药卫生—肿瘤]