原发性肝癌组织中CAIX蛋白的表达及其与微血管密度的关系  

Expression of carbonic anhydrase IX and its relationship with microvessel density in hepatocellular carcinoma

作　　者：赵建波[1] 王豪勋[2] 张玲[3] 赵晓静[1] 张中冕[2] 周士霞[2] 段芳龄[1] 马军[1] 

机构地区：[1]郑州大学第二附属医院消化内科,河南郑州450014 [2]郑州大学第二附属医院肿瘤内科,河南郑州450014 [3]河南省肿瘤医院肝胆外科

出　　处：《胃肠病学和肝病学杂志》2016年第7期806-809,共4页Chinese Journal of Gastroenterology and Hepatology

基　　金：河南省医学科技攻关计划项目;教育部留学人员科技活动项目择优资助[2006]321

摘　　要：目的研究原发性肝癌(hepatocellular carcinoma,HCC)中CAIX的表达及其与微血管密度(MVD)、临床病理特征之间的关系。方法采用组织芯片和免疫组化方法检测HCC和癌旁组织中CAIX和CD34表达。结果共收集41例HCC、36例癌旁组织,其中37例HCC和27例癌旁组织有病例资料。CAIX的表达在HCC和癌旁组织间差异无统计学意义(P>0.05),但HCC组织中CAIX包膜阳性表达率高于癌旁组织,而CAIX胞浆阳性表达率低于癌旁组织,差异有统计学意义(P<0.05)。HCC组织MVD明显高于癌旁组织(P<0.05)。CAIX阳性的HCC和癌旁组织中MVD均高于CAIX阴性患者,但只有癌旁组织中CAIX阳性组和阴性组间MVD差异有统计学意义(P<0.05)。结论 CAIX的包膜表达是HCC的特异特征;CAIX高表达HCC患者,血管再生活跃;CAIX可作为HCC治疗的潜在靶点。Objective To explore the expression of CAIX protein in hepatocellular carcinoma （HCC）and the rela- tionship of its expression with microvessel density（MVD） and clinic-pathological characteristics. Methods Immunohis- tochemistry was used to detect the expressions of CAIX and CD34 in tissue array of HCC. Results Forty-one HCC （37 cases with clinic-pathological data） and corresponding 36 para-cancerous tissue （27 cases with clinic-pathological data） were collected. No difference was detected for CAIX expression between HCC tissue para-cancerous tissue（P 〉0.05）. MVD（ calculated by CD34 expression） in HCC tissue was higher than that in para-cancerous tissue（P 〈 0.05）. MVDs of patients with positive CAIX expression both in HCC tissue and para-cancerous tissue were higher than those with CAIX negative patients, but difference was only found in para-cancerous tissue（P 〈 0.05）. Conclusion HCC is characteris- tic for cellular membrane expression for CAIX protein. HCC patients with higher CAIX protein expression are accompa- nied with up-regulated microvessel regeneration.

关 键 词：原发性肝癌 CAIX蛋白 微血管密度 

分 类 号：R735.7[医药卫生—肿瘤]