化疗后肝癌侵袭转移潜能的变化及其机理的实验研究  

The Mechanism of Potential Invasion and Metastasis in HCC after Chemotherapy

作　　者：刘俊[1] 孙秋实[2] 

机构地区：[1]湖北省仙桃市第一人民医院,433000 [2]湖北省襄阳市中心医院,441021

出　　处：《实用癌症杂志》2016年第7期1050-1052,共3页The Practical Journal of Cancer

摘　　要：目的探讨化疗后肝癌侵袭转移潜能的变化及其机理。方法运用基因芯片的方法筛查出人肝癌的相关因子,在筛查出的因子中进一步用免疫组化进行验证,化疗前和化疗后进行比较。由于化疗后肿瘤侵袭转移能力减弱,所以进一步检测其侵袭转移变化,通过免疫荧光检测进行确认。染色指标为LYVE-1(标记微淋巴管)和VEGF,检测样本化疗后组织,化疗前组织。结果检测临床样本中相关因子的表达,在检测结果中发现在化疗后VEGF低表达,为了确定这一结果,采用免疫组化进一步确定了这一结果,发现化疗前高表达VEGF,P<0.05。免疫荧光结果显示在低表达VEGF时的癌组织中淋巴管的表达也降低(相比于癌旁组织和正常组织)。结论化疗后VEGF可以通过调节淋巴内皮细胞进行抑制肝癌细胞的侵袭转移。Objective To study the mechanism of potential invasion and metastasis in HCC after chemotherapy. Methods Related gene from HCC tissues were screened by gene array,verificated by IHC,and compared after chemotherapy and before chemotherapy. Because of the decrease of potential invasion and metastasis after chemotherapy,the endothelial lymphatic were detected by IF,LYVE-1,the marker of lymphatic endothelial cell and VEGF was the marker of vascular endothelial cells. Results Expression of VEGF HCC after chemotherapy decreased,and compared with before chemotherapy,P〈0. 05,low expression of VEGF showed low lymph-vessel in cancer tissues. Conclusion VEGF can regulate the endothelial lymphatic to inhibit invasion and metastasis in HCC.

关 键 词：血管表皮生长因子 肝癌 淋巴管 化疗 

分 类 号：R735.7[医药卫生—肿瘤]