冷水浸泡对离心运动后大鼠骨骼肌卫星细胞的影响  

Effects of Cold Water Immersion on Skeletal Muscle Satellite Cellsin Rats After Eccentric Exercise

作　　者：张俊峰 毛旭娟[2] 吴丽君[3] 陈昊[4] 

机构地区：[1]山西体育职业学院社会体育系,山西太原030006 [2]山西职工医学院基础部病理生理教研室,山西太原030012 [3]山西大学体育学院,山西太原030006 [4]北京体育大学运动人体科学学院,北京100084

出　　处：《四川体育科学》2016年第4期38-41,54,共5页Sichuan Sports Science

摘　　要：目的:探究运动训练后冷水浸泡在促进机体功能恢复过程中是否会对肌卫星细胞产生影响。方法:68只雄性SD大鼠随机分为对照组(C)、运动组(E)、运动冷水浸泡组(CWI),并根据取材时间点再分为0h、24h、48h、72h、120h等时相亚组。E和CWI组进行跑台离心运动,其中CWI组运动后进行10℃冷水浸泡10min;蛋白免疫印记法检测腓肠肌Myo D、Myogenin蛋白表达。结果:Myo D蛋白在C、E、CWI组之间均有显著性差异(p<0.05),E组峰值出现在72h,CWI组峰值出现在48h;Myogenin蛋白在C和E、C和CWI组之间均有显著性差异(p<0.05),但E和CWI之间未有显著性差异(p>0.05),E组峰值出现在120h,CWI组峰值出现在72h。结论:离心运动后,大鼠骨骼肌Myo D和Myogenin的表达上调,运动后进行冷水浸泡能够加快肌卫星细胞的增殖分化,这可能是冷水浸泡促进机能恢复的部分原因。Objective： The aim of this study is to observe the effects of eccentric exercise and cold water immersion on the skeletal muscle satellite cells. Methods 68 male SD rats were randomly divided into control group （C）, exercise group （E） and exercise plus cold water immersion group （CWI） . Rats in groups E and CWI were submitted to downhill running on a treadmill. Rats in groups CWI received cold water immersion （10℃, 10min） therapy immediately after running. Protein expressions of Myo D, my ogenin were detected by western blot. Results： The MyoD protein was significantly different from group C, E ,CWI （P〈 0.05 ） ,and peaked at 72h in group E and 48h in group CWI; The Myogeninprotein was significantly different from group C and E ,C and CWI （P〈 0.05 ）, notsignificantly different from group E and CWI（p〉0.05 ）, and peaked at 120h in group E and 72h in group CWI. Conclusion eccentric exercise may up-regulate MyoD and myogeninprotein expression, to speed up the muscle satellite ceils proliferation and differentiation after cold water immersion, this is part of the reason that promoted the functional recovery.

关 键 词：冷水浸泡 离心运动 肌卫星细胞 增殖分化 

分 类 号：G804.7[文化科学—运动人体科学]