FABP-5基因沉默对人胶质瘤细胞增殖、侵袭、凋亡及肿瘤干细胞标志物CD44^+CD133^+表达的影响  

Effects of fatty acid binding protein-5 silencing on proliferation, apoptosis, invasion of human glioma cells and expressions of CD44^+ and CD133^+

作　　者：刘海鹏[1] 王冀伟[1] 郑克彬[1] 

机构地区：[1]河北大学附属医院神经外科,河北省保定市071000

出　　处：《中国组织工程研究》2016年第28期4142-4148,共7页Chinese Journal of Tissue Engineering Research

摘　　要：背景:已有研究表明,FABPs在多种恶性肿瘤,包括乳腺癌、前列腺癌、肝癌、肺癌、膀胱上皮癌都有不同程度的表达,与恶性肿瘤的发生、转移、侵袭和抗药性关系密切。目的:探讨沉默FABP-5基因表达对人胶质瘤细胞U251增殖、侵袭、凋亡的影响。方法:以人胶质瘤细胞U251细胞为研究对象,根据FABP-5 mR NA编码序列设计并合成干扰siR NA序列,瞬时转染U251细胞。将人胶质瘤细胞U251分为3组:FABP-5-shR NA组加入Lv-shR NA-FABP-5,阴性对照组加入LV-sh RNA-NC,空白对照组仅行常规培养。RT-PCR和Western blot法分别从mR NA和蛋白水平检测FABP-5的表达,CCK-8法测定细胞体外增殖能力,流式细胞仪检测细胞周期、细胞凋亡及肿瘤干细胞标志物CD44+CD133+的表达,TUNEL染色检测细胞凋亡。结果与结论:(1)与阴性对照组和空白对照组比较,FABP-5-sh RNA组的FABP-5 m RNA和蛋白表达明显下降;(2)与阴性对照组和空白对照组比较,FABP-5-shR NA组细胞生长速度明显减慢,细胞周期阻滞在G0/G1期,S期细胞数减少;肿瘤干细胞标志物CD44+CD133+的表达比例亦显著降低(P<0.05);(3)与阴性对照组和空白对照组相比,FABP-5-sh RNA组细胞凋亡率明显升高(P<0.01),增殖、侵袭力显著下降(P<0.05);(4)结果表明,FABP-5基因可能直接或间接地参与到胶质瘤细胞周期的调控和凋亡过程,其基因的表达水平改变与肿瘤细胞侵袭力关系密切。BACKGROUND：It has been demonstrated that there are different expressions of fatty acid binding proteins （FABPs） in most malignant tumors such as breast cancer, prostate cancer, liver cancer, lung carcinoma and bladder carcinoma;therefore, FABPs are closely related to the occurrence, metastasis, invasion and drug resistance of malignant tumors. OBJECTIVE：To investigate the effects of fatty acid binding protein-5 （FABP-5） silencing on the proliferation, invasion and apoptosis of U251 cel s （human glioma cel s）. METHODS：siRNA molecules targeting the mRNA of FABP-5 was designed and chenical y synthesized, which was transiently transfected into U251 cel s. U251 cel s were divided into three groups：Lv-shRNA-FABP-5 was added into FABP-5-shRNA group, LV-shRNA-NC added into negative control group, and blank control group underwent normal culture. mRNA and protein expressions of FABP-5 were detected by RT-PCR and western blot assay, respectively. The cel proliferation in vitro was determined by cel counting kit-8 assay, the cel cycle, apoptosis and expressions of CD44＋and CD133＋were detected by flow cytometry, and the apoptosis was observed using TUNEL staining. RESULTS AND COUNCLUSION：mRNA and protein expressions of FABP-5 in the FABP-5-shRNA group were significantly lower than those in the negative control and blank control groups. Compared with the negative control and blank control groups, the cel growth rate was significantly decreased, the cel cycle arrested in the G0/G1 phase, and the cel number in the S phase was decreased, and moreover, the proportion of CD44＋/CD133＋expression was significantly decreased in the FABP-5-shRNA group （P〈0.05）. Besides, compared with the negative control and blank control groups, the apoptosis rate was significantly increased, and the cel proliferation and invasiveness were significantly decreased in the FABP-5-shRNA group （P〈0.05）. In conclusion, it is possible that FABP-5 directly or indirectly regulates the cel cycle and apoptosis of glioma

关 键 词：肿瘤干细胞 神经胶质瘤 RNA干扰 脂肪酸结合蛋白质类 组织工程 干细胞 胶质瘤 表皮脂肪酸结合蛋白5 细胞周期 凋亡 

分 类 号：R394.2[医药卫生—医学遗传学]