骨髓间充质干细胞联合依达拉奉移植脑梗死大鼠血清炎性因子的表达  

Expression of serum inflammatory cytokines in rats with cerebral infarction undergoing bone marrow mesenchymal stem cell transplantation combined with edaravone

作　　者：宋乃光[1] 孙晶晶[2] 张耀龙[1] 孟令海[2] 高书焕 薛建[2] 孙彩月 

机构地区：[1]唐山市协和医院急诊科,河北省唐山市063000 [2]唐山市协和医院神经内科,河北省唐山市063000

出　　处：《中国组织工程研究》2016年第28期4162-4168,共7页Chinese Journal of Tissue Engineering Research

摘　　要：背景:抑制血清炎性因子凝血酶元激活因子的抑制因子1和组织纤维溶酶原激活物表达是治疗脑梗死的潜在靶点之一。目的:观察骨髓间充质干细胞移植联合依达拉奉对脑梗死大鼠血清炎性因子的表达变化。方法:60只SD大鼠采用大脑中动脉线栓法复制局灶性脑梗死模型后,随机等分为模型组、骨髓间充质干细胞移植组和联合组。模型组连续5 d尾静脉注射PBS,骨髓间充质干细胞移植组经尾静脉注射2.0×109 L-1骨髓间充质干细胞悬液1 mL,2次/d,连续5 d。联合组静脉滴注依达拉奉30 mg+尾静脉注射2.0×109 L-1骨髓间充质干细胞悬液1 mL(2次/d),连续5 d。结果与结论:(1)与模型组相比,骨髓间充质干细胞移植组与联合组大鼠m NSS评分较低,血清凝血酶元激活因子的抑制因子1表达水平减少,脑梗死面积减少,脑组织中肿瘤坏死因子αmR NA的表达水平减少;(2)联合组大鼠上述指标变化差异比骨髓间充质干细胞移植组大;(3)结果提示骨髓间充质干细胞移植联合依达拉奉有助于促进脑梗死损伤神经功能的恢复。BACKGROUND：To inhibit the expressions of prothrombin activator inhibitor 1 and tissue plasminogen activator is one potential target for the treatment of cerebral infarction. OBJECTIVE：To investigate the expressions of serum inflammatory cytokines in rats with cerebral infarction undergoing bone marrow mesenchymal stem cel transplantation combined with edaravone. METHODS：Sixty Sprague-Dawley rats were enrol ed to prepare models of focal cerebral infarction by middle cerebral artery occlusion, and were randomly divided into four groups. Rats were given intravenous injection of PBS via tail veins for 5 consecutive days as model group, rats were subjected to intravenous injection of 2.0×109/L bone marrow mesenchymal stem cel suspension （1 mL） via tail veins, twice daily for 5 days as stem cel transplantation group, and those were given intravenous injection of 30 mg edaravone combined with intravenous injection of 2.0×109/L bone marrow mesenchymal stem cel suspension （1 mL） via tail veins for 5 days, twice daily, as combined group. RESULTS AND CONCLUSION：Compared with the model group, modified neurologic severity scores were lower, expressions of serum prothrombin activator inhibitor 1 and tumor necrosis factor-αmRNA in the brain decreased, and the infarct area reduced in the stem cel transplantation and combined groups. And the changed levels of above indicators in the combined group were significantly larger than those of the stem cel transplantation group. In conclusion, combination of bone marrow mesenchymal stem cel transplantation with edaravone can promote neural function recovery after cerebral infarction.

关 键 词：梗塞 大脑中动脉 间质干细胞 组织工程 干细胞 移植 脑梗死 骨髓间充质干细胞 依达拉奉 血清炎性因子 凝血酶元激活因子的抑制因子1 组织纤维溶酶原激活物 

分 类 号：R394.2[医药卫生—医学遗传学]