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作 者:张桂通[1,2] 冯杰扬 刘佳[2] 张艳金[2] 相子民[2] 孟繁星[2] 孙天胜[1,2]
机构地区:[1]解放军医学院,北京市100853 [2]解放军北京军区总医院,北京市100700
出 处:《中国组织工程研究》2016年第28期4189-4195,共7页Chinese Journal of Tissue Engineering Research
基 金:国际科技合作项目(2010DFA31250);创伤后炎性反应与全身病发症的关系及预防策略研究~~
摘 要:背景:老年慢性阻塞性肺疾病患者髋部骨折术后死亡率明显高于单纯髋部骨折患者。近年来,这一问题受到越来越多的关注,但是由于机制不明,目前临床对于此类患者尚无有效的干预手段。目的:观察骨髓基质干细胞对老年慢性阻塞性肺疾病大鼠髋部骨折后急性期肺部炎症水平的影响。方法:12月龄雄性SD大鼠30只,暴露香烟箱4个月后,按随机数字表随机分为3组:熏烟组,熏烟+髋部骨折+生理盐水组,熏烟+髋部骨折+骨髓基质干细胞组,后2组制备髋部骨折模型。骨折后24 h,3组大鼠取相同肺下叶组织和外周血,进行病理切片与炎症指标分析。结果与结论:(1)老年慢性阻塞性肺疾病大鼠闭合性髋部骨折后,肺组织炎细胞浸润、黏液分泌、气管腔狭窄或闭塞;(2)骨折后肺组织与血清中白细胞介素6、肿瘤坏死因子α、白细胞介素10水平均有所增高;(3)静脉注射骨髓基质干细胞后,肺组织病理改变较生理盐水组减轻,促炎因子白细胞介素6、肿瘤坏死因子α水平低于生理盐水组,抗炎因子白细胞介素10水平高于生理盐水组,差异有显著性意义(P<0.05),血浆中炎症因子水平变化与肺组织相似;(4)结果表明,长期暴露香烟箱中的老年大鼠肺组织病理学有所改变,骨髓基质干细胞能缓解老年慢性阻塞性肺疾病大鼠髋部骨折后的急性肺损伤。BACKGROUND:The elderly hip fracture patients with chronic obstructive pulmonary disease (COPD) have a higher postoperative mortality than those only with hip fractures. In recent years, it has become an issue of concerns. Because the mechanism is unknown, however, there are no effective clinical interventions for these patients. OBJECTIVE:To observe the effect of bone marrow stromal stem cel s (BMSCs) on the level of pulmonary inflammation in aged rats with chronic obstructive pulmonary disease (COPD) after hip fractures. METHODS:Thirty male Sprague-Dawley rats, 12 months old, were exposed to smoking for 4 months and randomized into three groups, smoking, smoking+hip fracture+normal saline, smoking+hip fracture+BMSCs groups. Animal models of hip fracture were made in the latter two groups. Twenty-four hours after hip fracture, the lower lobe and peripheral blood samples were taken from al rats in the three groups, to evaluate the pathological changes of lung tissue and detect levels of inflammatory factors in the lung tissue and peripheral blood. RESULTS AND CONCLUSION:After closed hip fracture, aged COPD rats exhibited inflammatory cel infiltration, mucus secretion, airway stenosis or occlusion;the levels of interleukin-6, tumor necrosis factor-αand interleukin-10 in the lung tissue and peripheral blood were increased. After intravenous injection of BMSCs, the pathological changes of the lung tissue were reduced, and the levels of pro-inflammatory factors, tumor necrosis factor-αand interleukin-6, decreased, but the level of anti-inflammatory factor interleukin-10 further increased, which were significantly different from those in the normal saline group (P〈0.05). These findings indicate that BMSCs can relieve acute lung injury in aged COPD rats with hip fractures.
关 键 词:干细胞 移植 慢性阻塞性肺疾病 骨髓间充质干细胞 髋部骨折 炎症反应 急性肺损伤 白细胞介素6 肿瘤坏死因子α 白细胞介素10
分 类 号:R394.2[医药卫生—医学遗传学]
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