壳聚糖导管填充辛伐他汀／泊洛沙姆407水凝胶促进大鼠坐骨神经缺损后运动功能恢复  

Bridging Sciatic Nerve Defect with Simvastatin Delivered by Injectable Thermosensitive Pluronic F-127 Hydrogel Promotes Nerve Regeneration in Rats

作　　者：郭琦[1] 刘灿[2] 海宝[2] 马腾[2] 王红[2] 宋纯理[2] 徐迎胜[1] 

机构地区：[1]北京大学第三医院神经内科,北京100083 [2]北京大学第三医院神经骨科,北京100083

出　　处：《中国微创外科杂志》2016年第7期638-644,共7页Chinese Journal of Minimally Invasive Surgery

基　　金：国家自然科学基金(No.81171693,No.81100895);国家高技术研究发展计划(863计划)(No.SS2015AA020304)

摘　　要：目的探讨壳聚糖导管复合辛伐他汀/泊洛沙姆407水凝胶对大鼠坐骨神经缺损后运动功能恢复的影响。方法选取成年SD大鼠40只,随机分成壳聚糖导管组、壳聚糖导管复合辛伐他汀0 mg水凝胶组、壳聚糖导管复合辛伐他汀0.5 mg水凝胶组和壳聚糖导管复合辛伐他汀1 mg水凝胶组(前2组为对照组,后2组为辛伐他汀治疗组),每组10只,制作左侧坐骨神经10 mm缺损模型,用壳聚糖导管桥接缺损,其内填充不同浓度的辛伐他汀水凝胶。术后4、6、8、10周进行坐骨神经指数检测,术后10周进行神经电生理、荧光金逆行示踪、腓肠肌相对湿重、肌纤维面积百分比和运动终板形态检测,观察神经缺损后运动功能恢复情况。结果术后4、6、8、10周,辛伐他汀治疗组的坐骨神经指数均明显高于对照组(P<0.05);术后10周辛伐他汀治疗组复合肌肉动作电位的峰-峰值、运动神经传导速度、荧光金标记的阳性神经元数量、腓肠肌相对湿重、肌纤维面积百分比和运动终板的恢复均显著优于对照组(P<0.05)。结论壳聚糖导管复合辛伐他汀/泊洛沙姆407水凝胶能够促进外周神经损伤后的功能恢复。Objective To investigate effects of simvastatin/pluronic F-127 hydrogel combined with chitosan conduit on motor functional recovery of rats with sciatic nerve defects . Methods A total of 40 adult Sprague-Dawley rats were assigned randomly into four groups：chitosan conduit group and groups of the conduit filled with pluronic F-127 hydrogel containing 0 mg, 0.5 mg, and 1 mg simvastatin （ the first two groups were control groups while the last two groups as simvastatin treated groups ） , with 10 rats in each group.A 10 mm gap was produced in left side of sciatic nerves and then was repaired by the chitosan conduit filled with pluronic F -127 hydrogel containing different doses of simvastatin .At different periods after surgery , the functional recovery was evaluated by sciatic functional index （ SFI ） , electrophysiology , fluoro-gold retrograde tracing , the relative gastrocnemius muscle weight （ RGMW ） , the percentage area of muscle fibers and the morphology of motor endplates . Results At 4, 6, 8, and 10 weeks after surgery , values of SFI in simvastatin treated groups were significantly higher than those in the control groups （P〈0.05）.Similarly, at 10 weeks after surgery, the peak amplitude and motor nerve conduction velocity of compound muscle action potential （CMAP）, number of FG-labled neurons , RGMW, the percentage area of muscle fibers and the morphology of motor endplates in simvastatin treated groups were superior to those in the control groups （ P 〈0.05 ）. Conclusion These findings indicate that filling chitosan conduits with simvastatin/pluronic F-127 hydrogel can improve motor functional recovery in rats .

关 键 词：周围神经缺损 辛伐他汀 壳聚糖导管 泊洛沙姆407水凝胶 功能恢复 神经再支配 

分 类 号：R745.4[医药卫生—神经病学与精神病学]