槐耳浸膏逆转胃癌耐药细胞上皮间质转化的实验研究  

作　　者：徐志远[1] 程向东[1] 杜义安[2] 吕航[1] 张袁驌 陈佳辉[1] 

机构地区：[1]浙江中医药大学附属第一医院胃肠外科,310006 [2]浙江省肿瘤医院腹部肿瘤外科,310022

出　　处：《浙江临床医学》2016年第8期1378-1380,共3页Zhejiang Clinical Medical Journal

基　　金：浙江省中医药重点研究计划（2012ZZ002）;浙江省中医药管理局一般项目（2013ZA022.2015ZA090）

摘　　要：目的研究槐耳浸膏对胃癌耐药细胞（SGC7901／Fu）耐药性的逆转作用及对上皮间质转化（EMT）的影响。方法CCK-8法计算SGC7901／Fu细胞的耐药性；光镜观察细胞形态的改变；realtimeRT-PCR检测EMT相关标记物、转录因子的表达；Western．blot法检测snail．及Zeb2蛋白的表达；激光共聚焦显微镜观察E—cadherin表达及分布。结果0．1-0．5mg／m1浓度范围内，槐耳浸膏能增加sGc-7901／Fu细胞对5-Fu、DDP的敏感性；槐耳浸膏作用后sGc7901／Fu呈上皮样细胞形态改变，伴上皮表型标记物表达下调、间质表型标记物表达上调，侵袭能力增强。结论槐耳浸膏能部分逆转SGC．7901／Fu细胞的耐药性，其机制可能是通过调控EMT实现的。Objective To investigate the huaier＇s role in reversing chemoresistance and epithelial-mesenchymal transition （ EMT ） of chemo- resistant gastric cancer cells （ SGC7901/Fu ） . Methods CCK-8 method was used to determinate the reversal role of Huaier on the chemoresistance of SGC7901/Fu cells; the realtime RT-PCR was used to determine the expression level of EMT markers and EMT-related transcriptional factors; Western-blot was used to determine the protein level of snail and Zeb2; Confocal microscopy analysis was used to determine the change of expression and distribution of E-cadherin; Transwell analysis was used to the change of invasion ability. Results The combined used of Huaier, at 0.1 - 0.5 mg/ ml concentration, and DDP or 5-Fu could increase the chemo-sensitivity of SGC7901/Fu cells to DDP and 5-Fu; The use of Huaier on SGC7901/ Fu cells resulted in mesenchymal appearance, decreased expression of epithelial markers and increased expression of mesenchymal markers. Conclusion Huaier could partly reverse the chemoresistance of SGC7901/Fu cells, the underline mechanism might include regulating EMT process.

关 键 词：胃癌 化疗耐药 上皮间质转化 槐耳 

分 类 号：R735.2[医药卫生—肿瘤]