大气压冷等离子体活性因子分析及其对克雷伯氏菌细胞膜影响  

Composition of Reactive Species in Cold Plasma at Atmospheric Pressure and its Effect on Klebsiella Pneumoiae Cell Membrane Permeability

作　　者：董晓宇[1] 刘婷婷[1] 王萧[1] 任春生[2] 修志龙[3] 

机构地区：[1]大连大学生命科学与技术学院,辽宁大连116622 [2]大连理工大学物理与光电工程学院,辽宁大连116023 [3]大连理工大学生命科学与技术学院,辽宁大连116024

出　　处：《高校化学工程学报》2016年第4期878-885,共8页Journal of Chemical Engineering of Chinese Universities

基　　金：国家自然科学基金(21246012;21306015;21476032);辽宁省自然科学基金(201202006);辽宁省科学事业公益研究基金(2011005001);大连市科学技术基金(2012J21DW017)

摘　　要：使用电源电压为26 V交流电激发空气来产生大气压冷等离子体,分别处理样品0、1、2、3、4、5 min。使用发射光谱(OES)对等离子体的活性成分进行分析;采用光谱扫描多功能读数仪(SSMR)对克雷伯氏菌细胞内活性氧进行分析;利用荧光分光光度计(FS)分析克雷伯氏菌细胞膜电位和膜通透性。OES结果表明,在电源电压26 V,放电间隙3 mm时,大气压空气介质阻挡放电等离子体产生的活性因子主要是紫外线和活性氧自由基。SSMR结果显示,与对照相比,克雷伯氏菌细胞内活性氧浓度随着处理时间延长,呈现先升高,再降低趋势。FS结果表明,细胞膜电位升高,促使细胞膜超极化,从而提高细胞膜通透性。研究结果提示,大气压介质阻挡放电等离子体在生物化学工程与技术领域具有潜在的巨大应用价值。Cold plasma was generated using alternating current （26 V） to excite air under atmospheric pressure. The treatment time for each sample group was 0, 1, 2, 3, 4, 5 min, respectively. Plasma components were analyzed by optical emission spectroscopy （OES） and the reactive oxygen species in Klebsiella pneumoniae were investigated by a spectral scanning multimode reader （SSMR）. Moreover, membrane potential and membrane permeability of K. pneumoniae cells were monitored by a fluorescence spectrophotometer （FS）. The OES results show that there are many ultraviolet （UV） and reactive oxygen species （ROS） when using 26 V power voltage and 3 mm discharge gap. SSMR results indicate the ROS content in K. pneumoniae increases first, and then decreases with discharge time. The cell membrane potential in K. pneumoniae is improved, which leads to the hyperpolarization of cell membrane. FS results show that the cell membrane permeability is enhanced. These results suggest that the barrier discharge plasma at atmospheric pressure has enormous potential in biochemical engineering and technology.

关 键 词：大气压冷等离子体 活性因子 细胞膜通透性 克雷伯氏菌 

分 类 号：TQ923[轻工技术与工程—发酵工程]