大蒜素对胰腺癌细胞上皮间质化的影响  

Allicin Inhibits Epithelial-mesenchymal Transition of Human Pancreatic Cancer PANC-1 Cells

作　　者：贺川[1] 马笛[1] 巩平[1] 黄伟[1] 

机构地区：[1]石河子大学医学院第一附属医院,新疆石河子832000

出　　处：《中国实验方剂学杂志》2016年第14期130-134,共5页Chinese Journal of Experimental Traditional Medical Formulae

基　　金：上海市重点实验室开放课题项目(PC-2013-02)

摘　　要：目的:探讨大蒜素对胰腺癌细胞上皮间质转化(EMT)的影响。方法:常规培养胰腺癌细胞株PANC-1细胞,分别以10μg·L^(-1)转化生长因子-β_1(TGF-β_1),10μg·L^(-1)TGF-β_1+5 mg·L^(-1)大蒜素,5 mg·L^(-1)大蒜素处理PANC-1细胞,另设空白组,镜下观察细胞形态学改变。细胞划痕实验检测大蒜素是否能降低胰腺癌细胞的迁移能力;免疫荧光细胞化学法及免疫印迹法(Western blot)检测各组钙黏附蛋白-E(E-cadherin),波纹蛋白(Vimentin)的表达变化。结果:TGF-β_1能够刺激PANC-1细胞发生典型的EMT形态变化,并伴有E-cadherin表达的上调及Vimentin的下调。划痕实验表明大蒜素能降低胰腺癌PANC-1细胞的迁移能力。免疫荧光细胞化学法及Western blot结果显示,与空白组及TGF-β_1组比较,大蒜素组的E-cadherin表达明显上调(P<0.05),Vimentin明显下调(P<0.05),这表明大蒜素具有抑制PANC-1细胞产生EMT的效应。结论:大蒜素可抑制胰腺癌细胞发生EMT,进而可能在抑制肿瘤浸润与转移过程中发挥重要作用。Objective： To investigate the effect of allicin on epithelial-mesenchymal transition （EMT） of pancreatic cancer cells. Method： The PANC-1 cells were cultured and treated with 10 μg·L-1 transforming growth factor-ill （TGF-fll）, 10 μg·L-1 TGF--β1 ＋ 5μg·L-1 allicin, 5μg·L-1 allicin, respectively. Untreated cells were used for the normal control. Morphological changes were observed under inverted microscope. Wound scratch assay were performed to further identify if allicin could reduce the migration ability of PANC-1 ceils. The expressions of E-cadherin and Vimentin in pancreatic cancer cells were detected by immunocytochemistry and Western blot. Result： The TGF-β1 can make PANC-1 cells show typical EMT morphological changes, which were accompanied by up-regulation of E-cadherin expression and down-regulation of Vimentin. Wound scratch assay indicated that allicin could reduce the migration ability of PANC-1 cells. According to the results of immunocytochemistry and Western blot, compared with the control group and the TGF-β1 group, the protein expression of E-cadherin was up-regulated in the allicin group （ P 〈 0.05 ） , and that of aimentin was down- regulated （P 〈 0.05）. Conclusion： Allicin can inhibit the EMT of human pancreatic cancer PANC-1 cells, and may play an important role in the inhibition of tumor invasion and metastasis.

关 键 词：大蒜素 胰腺癌 上皮间质转化 E-钙黏蛋白 波形蛋白 迁移能力 

分 类 号：R285.5[医药卫生—中药学]