Ⅰ型人类免疫缺陷病毒包膜gp120与乙型肝炎病毒表面抗原免疫原性比较  

The comparison of the immunogenicity of human immunodeficiency virus type-1 envelope gpl20 and human hepatitis Bvitus surface antigen

作　　者：王明霞[1] 王甲业[1] 李妍[1] 陈文江[1] 田丹[1] 袁丽[1] 庄敏[1] 凌虹[1] 

机构地区：[1]哈尔滨医科大学医学微生物学教研室,黑龙江省感染与免疫重点实验室,黑龙江省感染与免疫创新团队,黑龙江省普通高校病原学重点实验室,150081

出　　处：《国际免疫学杂志》2016年第4期313-318,共6页International Journal of Immunology

基　　金：基金项目：国家自然科学基金（81271824）;黑龙江省卫生厅科研课题（2012-760）;黑龙江省教育厅科学技术研究项目（12541392）;黑龙江省博士后科研启动金（LBH-Q14123）

摘　　要：目的 比较Ⅰ型人类免疫缺陷病毒（HIV-1）及乙型肝炎病毒（HBV）包膜蛋白初次免疫及加强免疫后诱导产生抗体的规律,为提高HIV-1包膜蛋白诱导保护性抗体产生能力提供创新思路.方法 以10周龄雌性C57BL/6小鼠为动物模型,分别用HIV-1 06044毒株gp120三聚体（gp120T）、HBV表面抗原（HBsAg）蛋白与AddaVax佐剂免疫小鼠,背部皮下注射,共免疫3次,每次免疫间隔3周,第一、第二次免疫后7d和第三次免疫后3d、7d取血;第一次免疫后7d、第三次免疫后3d、7d取脾组织.用酶联免疫吸附实验（ELISA）及酶联免疫斑点实验（ELISpot）方法检测免疫小鼠血浆特异性结合抗体滴度及抗体分泌细胞（ASC）数量.结果 gp120T和HBsAg两种蛋白初次免疫后,动物均未产生明显的特异性抗体.两种蛋白加强免疫后特异性抗体水平明显升高,gp120T一次加强免疫及两次加强特异性抗体滴度逐渐升高,而HBsAg一次加强抗体滴度已经接近两次加强的水平.二次加强免疫后,gp120T和HBsAg免疫鼠脾脏特异性ASC数量差异不显著.结论 HIV-1包膜gp120T加强免疫诱导抗体水平达到高峰慢于HBsAg加强免疫,即加强免疫后gp120T诱导的回忆反应慢于HBsAg.Objective To compare the ability of human immunodeficiency virus type 1 （HIV-1） 06044 strain envelope gp120 trimer （gp120T） and Hepatitis B virus surface antigen （HBsAg） in eliciting specific antibodies,and to provide new clues in the improvement of immunogenicity of HIV-1 envelope glycoproteins.Methods Firstly,HIV-1 06044 gp120T and HBsAg proteins were prepared.Ten-week old female C57BL/c mice were immunized subcutaneously with gp120T or HBsAg for three times with a 3-week interval.At day 7 after the first （prime）,second immunization （boost 1） and at day 3 and day 7 after the third immunization （boost 2）,plasma and spleen single lymphocytes were prepared.Antigen-specific plasma antibodies were detected by enzyme linked immunosorbent assay （ELISA）.The antibody secreting cells （ASC） in spleen were measured by B-ELISpot.Results The specific antibody was undetectable in the mouse plasma after.the prime with gp120T and HBsAg.The antibody titers significantly increased following subsequent boosts.However,the antibody titers in gp120T immunized mice with the two boosts were increased gradually,while they were similar in the HBsAg immunized mice.ASGs in spleen raised significantly after the boosts at similar level in the two immnne groups.Conclusion The antibody titers after gp120T boosting reach high level slower than after HBsAg boosting indicating that gp120T boosts elicit slower specific recall responses than HBsAg.

关 键 词：Ⅰ型人类免疫缺陷病毒 GP120 乙型肝炎病毒 表面抗原 

分 类 号：R512.62[医药卫生—内科学]